Helixbind, Inc.

Alon Singer

Ranjit Prakash

Jork Nolling

David Steinmiller

Srinivas Rapireddy

Nadish Goyal

Fluidic devices and related methods are generally provided. The fluidic devices described herein may be useful, for example, for diagnostic purposes (e.g., detection of the presence of one or more disease causing bacteria in a patient sample). Unlike certain existing fluidic devices for diagnostic purposes, the fluidic devices and methods described herein may be useful for detecting the presence of numerous disease causing bacteria in a patient sample substantially simultaneously (e.g., in parallel). In some embodiments, the fluidic devices and methods described herein provide highly sensitive detection of microbes in relatively large fluidic samples (e.g., between 0.5 mL and about 5 mL), as compared to certain existing fluidic detection (e.g., microfluidic) devices and methods. In an exemplary embodiment, increased detection sensitivity of microbial pathogens present in a patient sample (e.g., blood) is performed by selectively removing human nucleic acid prior to sensitive detection of microbial infection. In some embodiments, the fluidic device allows for the identification of microbial pathogens directly from unprocessed blood without having to conduct blood culturing processes.

Integrated fluidic devices and related methods

The present disclosure generally relates to the field of microbial pathogen detection and identification utilizing genomic sequence recognition.

Methods and devices for identifying microbial infections

The present disclosure generally relates to the field of ultrasensitive microbial pathogen detection and identification utilizing genomic sequence recognition.

Methods and devices for ultrasensitive direct detection of microorganisms

Fluidic systems and reagent carriers suitable for storing reagents in a desirable manner are generally provided. In some embodiments, a reagent carrier stores a liquid film comprising a solid reagent and/or stores different reagents in different locations. In some embodiments, a fluidic system comprises a reagent carrier constrained such that it comprises an elongated axis positioned within 30&#xb0; of a vertical axis of the fluidic reservoir.

Reagent carriers for fluidic systems

Methods and devices for isolating microbial cells from a sample, extracting eukaryotic DNA from a sample, and identifying the microbial species in the sample are disclosed herein.

Methods and devices for detecting and identifying microorganisms

The present disclosure provides for compositions of &#x3b3;PNA probes. Additionally, the present disclosure provide for methods and kits using &#x3b3;PNA probes for the diagnosis of sepsis.

Methods and compositions for the diagnosis of sepsis using gamma peptide nucleic acids

The present disclosure provides for composition of &#x3b3;PNA probes. Additionally, the present disclosure provide for methods and kits using &#x3b3;PNA probes for the prognosis of sepsis. In some embodiments, wherein the &#x3b3;PNA capture probes and &#x3b3;PNA reporter probes comprise one or more functional moiety selected from the group consisting of: a binding molecule, a spacer group, a linker group, a hydrophobicity-changing group, a charge-inducing group, and a structural charge-inducing group.

Growing community of inventors

Waltham, MA, United States of America

Alon Singer