Sandoz Ag

Boehringer Ingelheim Rcv Gmbh & Co. Kg

Other

Biosepra Inc.

Novartis Ag

Takeda Pharmaceutical Company Limited

Novasep Process

Bia Separations D.o.o.

Boehringer Ingelheim Austria Gmbh

Alois A Jungbauer

Rainer Hahn

Alexandru Trefilov

Waltraud Kaar

Hermann W Katinger

Franz J Steindl

Hans Peter Lettner

Anne Tscheliessnig

Ales Strancar

Clemens Achmüller

Andrea Buchacher

Bernhard Auer

Milos Barut

Robert Schlegl

Ales Podgornik

Roman Necina

Philipp Wechner

Peter Satzer

Nikolaus Hammerschmidt

Anne-Luise Tscheliessnig

Christine Machold

Roland Grunow

Moritz Imendoerffer

Tomas Porstmann

Ruediger Von Baehr

Hannah Engelmaier

Ralf Sommer

Michael Seifert

This disclosure relates to a bottom section for being connected to an assembly for separating a solid component from a fluid. The assembly includes an inclined plate settler with at least one sedimentation channel for letting a solid component to be separated settle, said plate settler comprising a lower portion and an upper portion, wherein said at least one sedimentation channel extends from the lower portion to the upper portion. The bottom section is configured to be connected to the lower portion of the inclined plate settler. The bottom section comprises at least one inlet channel for feeding a fluid comprising the solid component to be separated to the plate settler, and at least one collection channel for collecting a settled solid component descending from the at least one sedimentation channel. Said at least one inlet channel and said at least one collection channel are fluidly separated from each other, said inlet channel and said collection channel being connectable to said at least one sedimentation channel, to form fluid connections between said at least one inlet channel and said at least one sedimentation channel and between said at least one collection channel and said at least one sedimentation channel, respectively.

Bottom section for being connected to an assembly with plate settler, and assembly with plate settler

The present invention provides novel methods of cell disruption and release of biomolecules from a cell. The invention comprises the use of positively and/or negatively charged microparticles comprising ground resin. It is particularly useful for purification of biomolecules from cell culture.

Microparticles for cell disruption and/or biomolecule recovery

The present invention provides a novel adsorbent composition for recovering biomolecules from a fluid. The composition comprises positively and negatively charged microparticles in the form of ground particles. The adsorbent is particularly useful for purification of biomolecules from the cell culture.

Adsorbent composition and use thereof

This application relates to methods for purification of proteins such as antibodies using a hydrophilic polymer (e.g., PEG), a fatty acid (e.g., caprylic acid), and/or another agent (e.g., calcium chloride).

Purification of proteins

This disclosure relates to methods for isolating antibodies from cell-free culture supernatant.

Methods for purification of antibodies using aliphatic alcohols

Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprises the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein the steps are conducted on one affinity chromatography system.

Production of recombinant proteins by autoproteolytic cleavage of a fusion protein

Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprising the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein said steps are conducted on one affinity chromatography system.

Disclosed is an affinity matrix comprising a solid phase and an affinity ligand comprising peptide bonds coupled to this solid phase, wherein the affinity ligand comprising peptide bond is selected from the following group of ligands: a) peptides comprising the formula XXXX, wherein Xto Xare amino acid residues and at least two of Xto Xis W, Y or F; b) peptides comprising the formula XXXX, wherein Xto Xare amino acid residues, at least one of Xto Xis W, and at least one of Xto Xis E or D; and c) poly-amino acids consisting of an amino acid monomer of the group consisting of R, K, E and D and an amino acid monomer of the group consisting of Y, F and W, preferably poly-KY, poly-KF, poly-KW, poly-RY, poly-RF, poly-RW, poly-EY, poly-DY, poly-EF, poly-EW, poly-DF and poly-DW, with the proviso that the peptides according to a) and b) have a maximum length of 35 amino acid residues and that the poly-amino acids according to c) have a minimum length of 20 amino acid residues.

Affinity ligands

In a method for reconstituting a recombinant protein from a denatured state to its active form, a feed solution containing the recombinant protein in its denatured and/or in biologically inactive intermediate forms is subjected to a chromatographic separation process, in which the protein is reconstituted under conditions that promote refolding of the protein and the intermediate forms are separated from the refolded protein. The denatured form and/or the inactive intermediate forms of the protein are separated from the refolded protein in a continuous or quasi-continuous manner and optionally recycled to the feed solution.

Method for reconstituting a recombinant protein to its biologically active form

Chromatography material and a process of manufacturing that material

Novel human monoclonal anti-HIV-I antibodies and immunochemicals made from the antibodies and method of treating or preventing HIV-I infections.

Human monoclonal anti-HIV-I-antibodies

The invention is directed to human monoclonal antibodies specific for the gp41 envelope protein of human immunodeficiency virus type 1 (HIV-1). Specifically disclosed are the human monoclonal antibodies produced by the cell lines 3D6 having ECACC Accession No. 87110301, 24G3 having ECACC Accession No. 90091702 and 25C2 having ECACC Accession No. 80120601.

A method for sterilizing liquid chromatography resins that are highly resistant to oxidation by strongly oxidizing agents and a sterilization solution for use therein. In a preferred embodiment, the invention comprises contacting an oxidant-resistant chromatographic resin with an aqueous solution of peracetic acid and up to 40% ethanol. In particular, where the solution is to be used to sterilize a positively charged ion-exchange resin, the solution preferably additionally includes an acetate buffer of a sufficient ionic strength to prevent adsorption of the peracetic acid ions on the resin.

Method for sterilizing liquid chromatography resins highly resistant to

A liquid chromatography column well-suited for in situ sterilization effected by washing with a sterilization solution. In a preferred embodiment, the column comprises a liquid chromatography tube packed with a liquid chromatography resin. A piston assembly is mounted within the liquid chromatography tube towards the upper end thereof, the piston assembly including an upper portion and a lower portion, the lower portion being shaped to include a fluid distribution channel and a downwardly-extending collar, the downwardly-extending collar including an annular slot and defining an orifice in fluid communication with the fluid distribution channel. The column further includes a distributor for distributing fluid conducted through the fluid distribution channel over the entirety of the orifice, the distributor being positioned over the liquid chromatography resin and a corrugated expanding ring for mounting the distributor within the orifice, the corrugated expanding ring being mounted in the annular slot and serving to support the distributor. Preferably, the distributor is a metal plate, either in the form of a multilayer sintered metal filter, a perforated plate with a hole diameter less than the lower grain diameter of the resin particles, or a woven and/or sintered stainless steel monolayer welded onto a metal ring.

Growing community of inventors

Vienna, Austria

Alois A Jungbauer