Harvard College

Vilnius University

University of Massachusetts

Allon Moshe Klein

David A Weitz

Marc W Kirschner

Linas Mazutis

Ilke Akartuna

John Heyman

Samuel Wolock

Huidan Zhang

Merav Socolovsky

Yung Hwang

Betsabeh K Tusi

Caleb Weinreb

The present invention generally relates to microfluidics and labeled nucleic acids. In one aspect, the present invention is generally directed to a method, wherein the method includes providing a plurality of droplets comprising particles, the particles comprising oligonucleotides, and attaching a nucleic acid sequence to the oligonucleotides. Certain embodiments are generally directed to systems and methods for splitting a droplet into two or more droplets. Certain embodiments are generally directed to systems and methods for sorting fluidic droplets in a liquid.

Barcoding systems and methods for gene sequencing and other applications

In certain aspects, disclosed herein are novel compositions and methods related to either the enhancement or inhibition of erythropoiesis that are useful, for example, in the treatment of anemia or erythrocytosis.

Methods and compositions for modulating erythropoiesis

The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include 'barcodes' or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.

Systems and methods for barcoding nucleic acids

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Boston, MA, United States of America

Allon Moshe Klein