Alliance for Sustainable Energy, LLC

Ut-Battelle, Inc.

Allison Jean Zimont Werner

Gregg Tyler Beckham

Christopher W Johnson

Adam Michael Guss

Daniel A Jacobson

Isabel Pardo Mendoza

Thelhawadigedara Lahiru Niroshan Jayakody

Thomas David Mand

Elsayed Tharwat Tolba Mohamed

Adam Michael Feist

Sandra Fabienne Notonier

Davinia Salvachúa Rodríguez

Erica Teixeira Prates

Disclosed herein are methods and compositions for catalytic glycolysis to deconstruct PET to bis(2-hydroxyethyl) terephthalate (BHET). For BHET conversion to terephthalate and ethylene glycol, we engineerKT2440 with PETase and MHETase enzymes from. We further engineerto convert terephthalate to a performance-advantaged bioproduct, β-ketoadipic acid, and for improved utilization of ethylene glycol, a byproduct of BHET catabolism. In a bioreactor, we produce 15.1±0.6 g/L of β-ketoadipic acid (βKA) from BHET at 76±3% molar yield. Lastly, we demonstrate conversion of catalytically depolymerized PET to βKA. Overall, this work highlights the potential of tandem catalytic deconstruction and biological conversion as a means to upcycle waste PET.

Engineered microorganisms for the deconstruction of polymers

The present disclosure relates to a genetically modified microbial cell that includes a first genetic modification resulting in the expression of an exogenous vanillate demethylase, such that the microbial cell is capable of metabolizing an S-lignin decomposition product and producing 2-pyrone-4,6-dicarboxylate (PDC).

Conversion of S-lignin compounds to useful intermediates

Disclosed herein are the genetically modifiedwith improved tolerance to hydroxycinnamic acids.

Microorganisms engineered for muconate production

Growing community of inventors

Denver, CO, United States of America

Allison Jean Zimont Werner