Corning Incorporated

Discovery Life Sciences, LLC

Allison Jean Tanner

Gregory Roger Martin

David A Kenney

William Joseph Lacey

Joseph Christopher Wall

Scott Matthew Bennett

Henry Joseph Cattadoris

Paul Michael Szlosek

Paul Ernest Gagnon, Jr

Todd M Upton

Ana Maria Del Pilar Pardo

Brian Robb Douglass

Vasiliy Nikolaevich Goral

Henry J Cattadoras

Qi Wu

Ye Fang

Ann MeeJin Ferrie

David M Root

Anthony Glenn Frutos

Christie Leigh McCarthy

Michael Kurt Schaefer

James George Clements

Hongming Wang

Timothy James Wood

Jeffry John Santman

Paul E Gagnon

Martin J Popoloski

John Wilfred Coddaire

Maryanne De Chambeau

Jessica Monique Brown

James Thomas Eickmann

Paula Mary Flaherty

Angela Langer Julien

Marshall Jay Kosovsky

Nicholas Michael Illsley

John Forrest Roush

John Shyu

Tora Ann-Beatrice Eline Sirkka

Kimberly Ann Titus

Ana Marie Del Pilar Pardo

Brent Ravaughn Lanterman

Alexander Meyer Wilks

Michael Curtis

Rongjun Zuo

Joseph C Wall

Feng Li

A cell culture apparatus includes a cell culture module including multiple cell culture chambers. A manifold connects the multiple cell culture chambers together along a side of the cell culture module. The manifold includes a side wall base structure connected to the side of the cell culture module and a column structure that is formed as a monolithic part of the side wall base structure. The column structure defines a fluid flow pathway through the manifold and to inlets to the cell culture chambers to allow filling and emptying of the cell culture chambers of liquid medium.

Cell culture apparatuses with manifolds including column structures

A fluidic device for culturing cells includes a microplate and plate lid. The microplate includes multiple wells and channels, the channels extending between the wells such that the channels interconnect the wells. The plate lid releasably engages the microplate to thereby enclose the wells and the channels. The wells include a culture surface such that a cell culture medium received therein is deposited over the culture surface. At least one channel that extends between adjacent ones of the wells is spaced from the culture surfaces of the adjacent wells defining a gap between the at least one channel and the culture surfaces of the adjacent wells for collection of the cell culture medium.

Fluidic devices including microplates with interconnected wells

A cell culture vessel has a wall and cell culture surface having a plurality of microcavities for culturing cells in three-dimensional conformation, referred to as 'spheroids'. The inner surface of the wall and the cell culture surface define a cell culture chamber of the vessel. The wall is attached to the cell culture surface in a way that does not provide flat surfaces on or around the cell culture surface so that the vessel provides an environment suitable for the production of a homogeneous population of three-dimensional cell clusters, or spheroids.

Cell culture vessel

A connected ecosystem for a laboratory environment comprises an electronic lab notebook, and instrumented biosafety cabinet, and one or more sensing vessels containing cell cultures. The electronic lab notebook interfaces with the instrumented biosafety cabinet to provide instructions, guidance, and monitoring of a user during the set up of the experimental protocol and to receive commands from the user via one of several input modalities. After the experimental protocol has been set up in the instrumented biosafety cabinet, cell cultures may be moved to an incubator where the connected ecosystem may provide automatic monitoring of the cultures. The automatic monitoring is provided by sensors integrated into cell culture vessels and supplemented by images of the cell cultures captured by a camera. The user may be informed of deviations from expected results detected based on the automatic monitoring.

Connected ecosystem for laboratory environment

A cell culture vessel includes a vessel body, support columns, and a stabilizer device. The vessel body defines a cell culture chamber enclosed between a bottom wall and a top wall. The support column is within the cell culture chamber and extends between the top wall and the bottom wall. The stabilizer device covers a width and length of the cell culture chamber and has a column engaging structure that is sized to slidingly engage the support column such that the stabilizer device is movable along the support column as a liquid culture medium is received in the cell culture chamber. The support column guides the stabilizer device along a length of the support column as the stabilizer device rises with rising liquid level in the cell culture chamber during a liquid culture medium filling operation.

Cell culture vessels with stabilizer devices

A cell culture apparatus may include a substrate defining a well. The well may define an interior surface, an exterior surface, an upper aperture and a nadir. The substrate may define a thickness between the interior and exterior surfaces that has a thickness proximate the nadir that is greater than or equal to a thickness proximate the upper aperture.

Microwell design and fabrication for generation of cell culture aggregates

A spheroid cell culture article including:

Spheroid cell culture article and methods thereof

A cell culture insert for use in culturing cells to promote the formation of spheroids and methods of using these spheroid-promoting cell culture inserts. The cell culture insert includes a porous membrane and one or more sidewalls that are non-adherent to cells and cause the cells in the insert to associate with each other and form spheroids.

Cell culture insert

This disclosure provides a cell culture media extending material capable of releasing nutrients into the cell culture environment slowly overtime. In embodiments, this material is a part of a cell culture vessel. In embodiments, the material is a coating or a film on a surface of a cell culture material. In additional embodiments, the material is a surface upon which cells are cultured, such as a cell culture vessel or a microcarrier.

Cell culture media extending materials and methods

A microcavity dish () for cultivating cells includes a dish body including a sidewall () that encloses a cell culture chamber within the dish body. The dish body has a top and a bottom (). The bottom includes a cell culturing substrate comprising an array of microcavities (). The sidewall includes a transition portion () that divides the sidewall into an upper portion and a lower portion that is offset inward relative to the upper portion defining a liquid medium delivery surface () that extends at least partially along an interior surface () of the sidewall and slopes toward the bottom.

Microcavity dishes with sidewall including liquid medium delivery surface

A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

Multilayered cell culture apparatus

A cell separation device configured for separating cells from microcarriers or spheroids in a liquid is provided. The cell separation device includes a vessel comprising a first port, a second port, and a cavity; and a porous mesh disposed within the cavity to divide the cavity into a first compartment and a second compartment, wherein the first port is in communication with the first compartment of the cavity, the first port located to a first side of the porous mesh, wherein the second port is in communication with the second compartment of the cavity, the second port located to a second side of the porous mesh, and wherein the porous mesh is positioned within the cavity to have a substantially vertical orientation or an inclined orientation with respect to a flow of liquid through the porous mesh.

Cell separation device and method for using same

A cell culture vessel () includes a wall including an inner surface defining a cell culture chamber of the vessel. A substrate () of non-porous material is positioned in the cell culture chamber between a first region of the cell culture chamber and a second region of the cell culture chamber. The substrate includes a plurality of microcavities (), each microcavity of the plurality of microcavities includes a concave surface defining a well and an opening, the concave surface of each microcavity includes at least one aperture including a dimension less than or equal to about 15 microns defining a path from the well to the second region. A cell culture vessel including a substrate including a plurality of microcavities and a layer of porous material is also provided. Methods of culturing cells in the cell culture vessel are also provided.

Growing community of inventors

Portsmouth, NH, United States of America

Allison Jean Tanner