University of Minnesota

Allison Hubel

Kathryn Lindsay Pollock

John Jeffrey McCullough

Jacob Hanna

Scott Robert Burger

Alptekin Aksan

Eduardo Reategui

This disclosure describes a cryopreservative composition and methods for storing cells. Generally, the cryopreservative composition includes a sugar component and a sugar alcohol component, and is effective for storing and recovering cells without requiring dimethyl sulfoxide (DMSO).

Cryopreservative compositions and methods

An example includes an apparatus for separating cells from a fluid sample, including a plenum, defining: a centrally located top inlet, and respective side outlets disposed below the inlet and to the side of the inlet; and a bottom outlet disposed below the top inlet, to the side of the two or more outlets, wherein the plenum is configured to receive a fluid and cells suspension through the inlet, and direct it to the bottom outlet, against respective side-walls extending between the bottom outlet and the two or more side outlets, and wherein the distance between each of the two or more side outlets and the bottom outlet is selected to encourage cells to exit through the bottom outlet under a force. Lateral/vertical distance between the inlet and side outlets can provide lateral fluid travel, thereby allowing time for gravity to bias cells toward an outlet.

Inlet and outlet geometries for a vertical three-stream microfluidic device

Disclosed herein are synthetic silica-based ocular devices fabricated from a composite material comprising silica and a fibrillar protein, together with methods of making and using the ocular devices.

Silica-based composite ocular device and methods

A composition and method for cryopreserving cells in a non-toxic medium is described. The composition and method provide cells for cell therapy and other in vivo applications.

Compositions and methods for cryopreservation of peripheral blood lymphocytes

An infusible-grade storage medium that is capable of preserving the viability and function of stem cells, nucleated cells and other hematopoietic cells is provided.

Growing community of inventors

Saint Paul, MN, United States of America

Allison Hubel