Monsanto Technology LLC

Other

Allen T Christian

James Arthur Baum

Haitao Zhang

Artem G Evdokimov

Farhad Moshiri

Lisa Marie Weaver

Kevin Lee Deppermann

G David Grothaus

Larry A Gilbertson

Michael W Petersen

Edward James Cargill

Andrew M Singleton

Brad D White

Jianping Xu

Mingqi Deng

R Douglas Sammons

Elias J Yannakakis

Ramesh Govinthasamy

Ryan K Bartlett

Sonya J Franklin

James J Schaefer

William A Grote

Chad Allen Stendal

Tiffany L Stephans

Donald W Mittanck

Yelena A Dudin

Artem Evdokimov

Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

Compositions and methods for the improved production and delivery of RNA by efficient transcription termination

Compositions and methods for the production and delivery of double stranded RNA

Portable systems and methods for amplifying nucleotides and for detecting nucleotide sequences in a sample are provided. The portable instruments and methods use RPA techniques for DNA amplification and detect sample fluorescence in response to amplification and/or to the presence of specific DNA sequences.

Portable systems and methods for amplifying nucleotides and detecting nucleotide sequences

The present invention provides methods of detecting for the presence of a polynucleotide in vivo. These methods are particularly useful for performing identification and/or analysis of samples or specimens in which it is impossible, impractical, or undesirable to move or remove them from their current environment. Methods of practicing the present invention for the purpose of identifying and/or analyzing transgenic plant tissue or cells, in addition to animal tissue or cells and bacterial cells are also provided.

Methods of polynucleotide detection

A high-throughput method is provided for analyzing individual seeds in a population of seeds. The method generally includes removing a tissue sample from at least one or more seeds in the population of seeds while preserving germination viability of the at least one or more seeds from which the tissue sample is taken, depositing the tissue sample into an individual compartment of a sample tray, contacting the tissue sample with an extraction buffer to remove DNA from the sample, and analyzing the extracted DNA for the presence or absence of a transgene of interest. The extraction buffer may be present in the individual compartment of the sample tray before the tissue sample is deposited into the individual compartment.

High throughput methods for sampling seeds

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Wildwood, MO, United States of America

Allen T Christian