Singular Genomics Systems, Inc.

Allen Lipson

Daan Witters

Eli N Glezer

Ryan Shultzaberger

Disclosed herein, inter alia, are novel methods pertaining to nucleic acid amplification and detection. Devices, compositions, and kits for use in such methods are also provided.

Chemical and thermal assisted nucleic acid amplification methods

Disclosed herein, inter alia, are methods of detecting or sequencing circular template polynucleotides on a solid support and/or in cells and tissues.

Methods of sequencing circular template polynucleotides

Disclosed herein, inter alia, are complexes, kits, and efficient methods of sequencing two strands of a double stranded polynucleotide.

Methods of amplifying circular polynucleotides in situ

Disclosed herein, inter alia, are polynucleotides, supports, kits, and methods of use thereof for amplifying, immobilizing, and sequencing polynucleotides.

Amplification oligonucleotides

Disclosed herein, inter alia, are substrates, kits, and efficient methods of preparing and sequencing two or more regions of a double-stranded polynucleotide.

Methods and compositions for sequencing complementary polynucleotides

Disclosed herein, inter alia, are complexes, kits, and efficient methods of sequencing two strands of a double stranded polynucleotide. In embodiments, methods of sequencing a circular template polynucleotide are provided including generating immobilized extension products.

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San Diego, CA, United States of America

Allen Lipson