Cryobiophysica, Inc.

Other

Organ, Inc.

Henry M. Jackson Foundation

Henry M. Jackson Foundation for the Military Medicine

Allen Gene Hirsh

Latchezar I Tsonev

Patrick M Mehl

Serguei Litvinovitch

The invention described here provides a novel method of preventing the loss of focus and range of colors when digital image files are expanded to a larger size. Unlike interpolation algorithms and algorithms using Fourier analysis to create new pixels, the present system uses a combination of pseudo-randomizing functions and user controlled edge detection to enhance edges initially softened by expansion using interpolation algorithms. The resulting images can be optimized to provide a superior approximation to what would be an accurate photographic representation at the larger image size. Furthermore, the flexibility in edge detection sensitivity allows the operator to strongly mitigate the structural artifacts produced by lossy compression algorithms such as the jpeg system and sampling errors widespread in very small images. As a result files as small as 200 kilobytes can be effectively expanded to 250 or even 500 megabytes in many cases.

Method for retaining edges in an enlarged digital image

Systems and methods for separating charged molecules on the basis of their differential electrostatic binding to a charged stationary phase comprise controlling the creation of simultaneous independent or dependent pH and additive gradients.

Multi-component, simultaneous, independent multi-gradient system for liquid chromatography

The invention provides novel methods for the separation of charged molecules such as proteins according to the isoelectric points (pI's) and includes the systems and buffering compositions employed for isolating charged molecules. The invention further provides for modifications to the above described chromatographic methods that enable the separation of charged molecules exhibiting virtually identical pI's by shifting both the buffer's pKa and the pI's of the eluted charged molecules while they are traversing the ion exchange column.

External gradient chromatofocusing

The present invention provides a new analysis based on Differential Spectral Topographic Analysis (DISTA). Using data from the spectral methods known in the art, DISTA is based upon the normalization of the spectra to a fixed topographic space, creating a set of spectral forms, and the summation of the absolute differences in topography between one or more reference spectra and the test spectra taken at different magnitudes of the perturbing parameter. This method allows for a sensitive estimate of the fraction of form A and form B of an entity of interest. This method also allows for the calculation of apparent free energy from the conversion of the entity of interest from a first to a second form where appropriate, and in the alternative, calculation of a fraction of structural changes.

Differential spectral topographic analysis (DISTA)

An apparatus and method for modified intrinsic state spectral analysis determines a proportion of a plurality of intrinsic structures of an optically active molecule. A spectropolarimeter generates a plurality of intrinsic spectra. Each of the plurality of intrinsic spectra is an intrinsic state vector corresponding to a plurality of intrinsic molecular structures. The spectropolarimeter also generates a spectrum for a sample of the optically active molecule. The sample spectrum is a sample state vector of the optically active molecule. A controller determines a plurality of estimate state vectors based on a plurality of perturbed intrinsic state vectors. Each of the plurality of perturbed intrinsic state vectors corresponds to a perturbed intrinsic structure. An optimum proportion of the plurality of perturbed intrinsic state vectors corresponds to each estimate state vector. The plurality estimate state vectors are matched against the sample state vector to determine a best match estimate state vector. The optimum proportion of the best matched estimate state vector is the proportion of the perturbed intrinsic molecular structures in the sample of the optically active molecule.

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Silver Spring, MD, United States of America

Allen Gene Hirsh