University of Alberta

Arcadia Biosciences, Inc.

Bayer Intellectual Property Gmbh

Bayer Cropscience Gmbh

Allen G Good

Virginia L Stroeher

Douglas G Muench

Ashok K Shrawat

Mary DePauw

Jean C Kridl

Perrin Hudson Beatty

George Theodoris

Yee Ying Lock

Chandra McAllister

Jayne D'Entremont

Mary Depauw

A transgenic plant comprising a polynucleotide encoding a nitrogen utilization protein operably linked to a PBpr1 promoter is provided. The transgenic plant exhibits increased nitrogen use efficiency, increased biomass and/or increased seed yield. Seeds from such transgenic plants, genetic constructs to prepare such plants, methods of generating and growing transgenic plants are also provided.

Plants having enhanced nitrogen efficiency

Methods are provided for enhancing yield and nitrogen use efficiency in plants, and to methods of increasing biomass and seed yield in plants grown under nitrogen limiting conditions using variants of enzymes involved in nitrogen assimilation or metabolism from non-plant organisms.

Plants having enhanced nitrogen use efficiency and methods of producing same

Methods of increasing nitrogen utilization efficiency in monocot plants through genetic modification to increase the levels of alanine aminotransferase expression and plants produced there from are described. In particular, methods for increasing the biomass and yield of transgenic monocot plants grown under nitrogen limiting conditions compared to non-transgenic plants are described. In this way, monocot plants may be produced that maintain a desired yield while reducing the need for high levels of nitrogen application.

Nitrogen-efficient monocot plants

Transgenic plants containing recombinant barley alanine aminotransferase are described. Also provided are methods for generating transgenic plants containing recombinant barley alanine aminotransferase.

Transgenic plants expressing recombinant barley alanine aminotransferase

Methods are provided by whichplants and seeds thereof may be modified to express a coding region of interest using a promoter sequence operatively linked to the coding region. The promoter sequence is an isolatedantiquitin (OsAnt1) promoter sequence including SEQ ID NO: 1. The coding region of interest may encode a nitrogen utilization protein, suitably alanine aminotransferase. Methods to developplants that have increased biomass and seed yield are also presented. Furthermore,plants may be produced that maintain a desired yield while reducing the need for high levels of nitrogen application.

Promoter sequence obtained from rice and methods of use

The present invention relates to non-naturally occurring plants that display enhanced agronomic characteristics due to elevated levels of nitrogen utilization proteins in the root of the plants. In particular, the present invention relates to non-naturally occurring plants with elevated levels of nitrogen utilization proteins specifically localized to the root epidermis of the plant. In addition, the present invention includes a preferred method of generating such non-naturally occurring plants by introducing into the plant a transgene encoding a nitrogen utilization protein operably linked to a root-epidermis-specific promoter.

Plants with enhanced levels of nitrogen utilization proteins in their root epidermis and uses thereof

A genetic construct is taught which contains a nitrogen assimilation and/or metabolism gene and a promoter for the gene which is inducible under conditions where it would be beneficial to take-up, store or use nitrogen. The promoter can be, for example, induced by the presence of nitrate or other form of nitrogen and will be induced by application of a nitrogenous fertilizer. The stress inducible promoter btg-26 is also taught. Promoter btg-26 is isolated from the Brassica turgor gene--26 and exhibits osmotic-stress induced expression.

Growing community of inventors

Edmonton, Canada

Allen G Good