Advanced Liquid Logic, Inc.

Duke University

Illumina Corporation

Other

The University of North Carolina at Chapel Hill

Illumina Cambridge Limited

Molecular Devices Corporation

Aerie Pharmaceuticals, Inc.

Xanthon, Inc.

Illumina Singapore Pte. Ltd.

Allen E Eckhardt

Vamsee K Pamula

Michael G Pollack

Vijay Srinivasan

Ramakrishna Sista

Richard B Fair

H Holden Thorp

Zhishan Hua

Jonathan Mark Boutell

Roberto Rigatti

Niall Anthony Gormley

Philip Y Paik

Arjun Sudarsan

Prasanna Krishnan Thwar

Alexander Shenderov

Rigo Pantoja

Petr Capek

Michael Hauser

Mitchell Anthony deLong

Cyril Delattre

Thomas E Richardson

Eric Hans Vermaas

Mary E Napier

Robert H Oakley

Dwayne J Allen

Sean M Ramirez

Brian H Heasley

Deborah John Boles

Marcos Sznaidman

Theodore M Winger

Uichong B Yi

Michael Robert Peel

John Groelke

Yin Nah Teo

Carson R Loomis

David Harris Stewart

Gregory F Smith

Edwin Li

Nicholas Trotta

Carrie A Graham

Jeremy Rouse

Hon Lung Chu

Robert S Thomas

Jill C Mikulecky

Zhihong Zhang

Christine Hudson

Jeffrey David Yingling

Xiangyuan Yang

Shuntai Wang

Raveendra Dayam

Mitchell A Delong

Paresma R Patel

Claire Louise Murray

Yangbin Gao

Byappanahally N Narasinga Rao

Rachel M Schowalter

Jimmy Perrott

Tong Wang

Yalin Xiong

Ning Wu

Sanjay Saha

Gajendrasinh Raolji

Lisa Perkins

Jonathan Benton

Scott Norton

Rahul Dhopeshwarkar

Jeffrey D Yingling

Peter Linnartz

Carrie Graham

Gajendrasinh Balvantsinh Raolji

Daniel Ortiz

Niall Gormley

Marcos L Sznaidman

A method comprising effecting a change in a shape of a droplet, wherein the droplet is disposed over a substrate in sensing proximity to a sensor and the droplet has a starting surface area exposed to the sensor; and producing an expanded surface area of the droplet in the sensing proximity exposed to the sensor, wherein the expanded surface area exposed to the sensor is greater than the starting surface area exposed to the sensor.

Droplet actuator devices and methods employing magnetic beads

Provided herein are methods of splitting droplets containing magnetically responsive beads in a droplet actuator. A droplet actuator having a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at the droplet operations electrodes, is provided. The magnetically responsive beads in the droplet are immobilized using the magnetic field and the plurality of droplet operations electrodes are used to split the droplet into first and second droplets while the magnetically responsive beads remain substantially immobilized.

Manipulation of beads in droplets and methods for manipulating droplets

Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.

Glucose-6-phosphate dehydrogenase assays

Embodiments of present application are directed to microfluidic devices and particularly digital micro-plastic fluidic devices that are specifically designed to prevent sample contamination during sample processing, methods of manufacturing the same, and methods to improve sample analysis process by preventing sample contamination.

Cationic polymers and method of surface application

A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged for conducting droplet operations on a surface; (ii) a starting droplet comprising: (1) one or more magnetically responsive beads; (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone; (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet substantially lacking the magnetically responsive beads.

The present disclosure generally relates to nucleic acid amplification systems and methods suitable for construction of nucleic acid samples, including construction of normalized nucleic acid libraries. In some embodiments, the method includes providing one or more input nucleic acid samples, contacting each of the input nucleic acid samples (e.g., input library) with a reaction mixture including first amplification or normalization primers and second amplification or normalization primers, wherein the first amplification or normalization primers are immobilized on a solid support and the second amplification or normalization primers are in solution phase, and amplifying the input nucleic acid samples under conditions such that substantially all of the first amplification or normalization primers are incorporated into amplification products. Further provided are systems and droplet actuator devices that are configured to carry out the methods disclosed herein. Compositions that include nucleic acid samples and libraries, preferably normalized, prepared in accordance with the disclosed methods and systems are also provided.

Methods and systems for construction of normalized nucleic acid libraries

Some embodiments disclosed herein provide methods of reducing reactive molecular species in a reaction carried out in a digital fluidics device having one or more electrodes by using a reaction mixture comprising an electron acceptor additive. In some embodiments, the presence of the electron acceptor additive reduces the presence of reactive molecular species in the reaction mixture. In some embodiments, the presence of the electron acceptor additive reduces the formation of gas bubbles in the digital fluidic device. Therefore, in preferred embodiments, the electron acceptor additive does not form a gas when reduced.

Methods of conducting biochemical reactions while reducing reactive molecular species during electrowetting

The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.

The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.

Integrated droplet actuator for gel electrophoresis and molecular analysis

The present disclosure relates to the field of molecular biology and more specifically to methods for capturing and amplifying target polynucleotides on a solid surface.

Polynucleotide modification on solid support

The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.

Droplet actuator and method

The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties.

Enzyme assays on a droplet actuator

Growing community of inventors

Durham, NC, United States of America

Allen E Eckhardt