Amersham Biosciences Ab

Pharmacia Biotech Ab

Amersham Pharmacia Biotech Ab

Allan Asp

Peder Carstenius

Ulf Landegren

Bengt-Goran Andersson

An apparatus for detecting fluorophore labelled substances in an electrophoretic separation medium comprises illuminating means ( ) for illuminating the fluorophore labelled substances ( ), detecting means ( ) for detecting fluorescence emitted by said fluorophore labelled substances upon illumination, and illumination varying means ( ) for varying the illumination of the fluorophore labelled substances ( ) to vary the intensity of the fluorescence.

Method and apparatus for detecting substances

A method of analyzing a sequence of a polynucleotide of interest, comprising the steps of: a) incorporating one member of a specific binding pair at the end of each strand of a double stranded polynucleotide of interest, the number being of the same type for both strands, b) immobilizing both strands of the polynucleotide to a solid support provided with the other member of the specific binding pair, c) annealing sequencing primers to the immobilized strands, d) sequencing both strands by the chain termination method. The polynucleotide of interest is preferably amplified before or in connection with step a) and most preferably by polymerase chain reaction extension. The invention also comprises a kit for use in analyzing the sequence of a polynucleotide of interest.

Method of sequencing

Method of nucleic acid transfer

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Uppsala, Sweden

Allan Asp