Cellfe, Inc.

Alla Zamarayeva

Alexander Alexeev

Sewoon Han

Ian Sicher

Miguel Calero-garcia

The present disclosure provides methods and systems for cell processing, including delivery of imaging agents into cells. The methods and systems may comprise the use of a microfluidic device. The microfluidic device may comprise a channel comprising a compressive element. The compressive element may be configured to reduce a volume of the cell and facilitate the formation of one or more transient pores in a cell membrane of the cell. The one or more pores may permit one or more imaging agents to enter the cell. Also provided are modified cells produced using the disclosed methods and systems and methods of imaging the modified cells in a subject.

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Berkeley, CA, United States of America

Alla Zamarayeva