Ajinomoto Co., Ltd.

Alla Lvovna Lapidus

Nelli Isaakovna Zhdanova

Vladimir Georgievich Debabov

Nikolai Kazimirovich Yankovsky

Andrei Yurevich Christoserdov

Alexander Konstantinovich Sokolov

Michail Markovich Gusyatiner

Uri Dmitrievich Tsigankov

Vytalyi Arkadievich Lifshits

Tatiana Alexandrovna Bachina

Sergi Vladimirovich Mashko

Evgenyi Moiseevich Khurges

Oksana Fedorovna Gavrilova

Uri Ivanovich Kozlov

Oleg Alexandrovich Rodionov

The present invention provides a novel microbial strain and a method for effectively producing L-threonine. The novel strain can grow on a medium containing molasses, a much cheaper raw material than sucrose. The exemplary novel strain E. coli BKIIM B-5318 bears the plasmid pPRT614, which has threonine biosynthesis genes (thr A, B and C), the expression of which is regulated by a lambda-phage PR promoter and temperature-sensitive C1 repressor. The present microorganism is prototrophic with regard to isoleucine. The strain E. coli BKIIM B-5318 produces more than 70 g/l of L-threonine when cultured at 38-41.degree. C. for 32 hours in a medium containing molasses.

L-threonine-producing microbacteria and a method for the production of

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Moscow, Russia

Alla Lvovna Lapidus