University of Massachusetts

Carnegie Institution of Washington

Juanita Mclachlan

Phillip D. Zamore

Craig C. Mello

Alla Grishok

Gyorgy Hutvagner

Craig C Mello

Phillip D Zamore

Juanita McLachlan

Andrew Fire

Hiroaki Tabara

Gyoergy Hutvagner

Philip D Zamore

György HUTVÁGNER

Gyorgy Hutvanger

The invention provides engineered RNA precursors that when expressed in a cell are processed by the cell to produce targeted small interfering RNAs (siRNAs) that selectively silence targeted genes (by cleaving specific mRNAs) using the cell's own RNA interference (RNAi) pathway. By introducing nucleic acid molecules that encode these engineered RNA precursors into cells in vivo with appropriate regulatory sequences, expression of the engineered RNA precursors can be selectively controlled both temporally and spatially, i.e., at particular times and/or in particular tissues, organs, or cells.

In vivo production of small interfering RNAs that mediate gene silencing

In vivo production of small interfering RNAS that mediate gene silencing

Vivo production of small interfering RNAs that mediate gene silencing

The invention provides engineered RNA precursors that when expressed in a cell are processed by the cell to produce targeted small interfering RNAs (siRNAs) that selectively silence targeted genes (by cleaving specific mRNAs) using the cell's own RNA interference (RNAi) pathway. By introducing nucleic acid molecules that encode these engineered RNA precursors into cells in vitro with appropriate regulatory sequences, expression of the engineered RNA precursors can be selectively controlled both temporally and spatially, i.e., at particular times and/or in particular tissues, organs, or cells.

Genes involved in double-stranded RNA interference (RNAi pathway genes) are identified and used to investigate the RNAi pathway. The genes and their products are also useful for modulating RNAi pathway activity.

RNA interference pathway genes as tools for targeted genetic interference

Growing community of inventors

New York, NY, United States of America

Alla Grishok