Wilson Wolf Manufacturing Corporation

Wheaton Industries, Inc.

Alison Robeck

John R Wilson

Douglas A Page

Daniel P Welch

Dan Welch

Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.

Cell culture methods and devices utilizing gas permeable materials

A versatile compartmentalized cell culture device, with a selectively permeable membrane separating the compartments, provides many attributes relative to traditional devices. It can be configured for high-density cell culture, co-culture, and sample dialysis while rolling or standing still. It can also be configured for continuous movement of liquid between compartments. The wide combination of attributes not found in other membrane based cell culture and bioprocessing devices includes more cell capacity, more cell secreted product capacity, higher cell and product density, increased medium capacity, minimized use of exogenous growth factors, compatibility with standard cell culture equipment and protocols, increased scale up efficiency, capacity to function when rolling or standing still, capacity for perfusion without the need for pumps, and more efficient sample dialysis.

Culturing cells in a compartmentalized roller bottle

Compartmentalized device for cell culture, cell processing, and sample dialysis

Growing community of inventors

Monticello, MN, United States of America

Alison Robeck