University of Vienna

Alipasha Vaziri

Robert Prevedel

Tobias Nöbauer

Devices and methods for recording dynamics of cellular and/or biochemical processes, including a device including one or more dispersive elements configured to receive a pulsed laser beam with a spectrum of different wavelengths and disperse the spectrum of the pulsed laser beam; and one or more first elements configured to receive the dispersed spectrum of the pulsed laser beam, and generate a multiphoton excitation area in a biological sample by re-overlapping in time and space the dispersed spectrum of the pulsed laser beam on an area in the biological sample, wherein the device is configured to record at high speed changes of cellular and biochemical processes of a population of cells of the biological sample based on generation of the multiphoton excitation area in the biological sample.

Recording dynamics of cellular processes

Methods and systems are provided for enhancing the imaging resolution of three dimensional imaging using light field microscopy. A first approach enhances the imaging resolution by modeling the scattering of light that occurs in an imaging target, and using the model to account for the effects of light scattering when de-convolving the light field information to retrieve the 3-dimensional (3D) volumetric information of the imaging target. A second approach enhances the imaging resolution by using a second imaging modality such as two-photon, multi-photon, or confocal excitation microscopy to determine the locations of individual neurons, and using the known neuron locations to enhance the extraction of time series signals from the light field microscopy data.

Growing community of inventors

Vienna, Austria

Alipasha Vaziri