Asahi Glass Company, Limited

Agc Inc.

Alimjan Idiris

Hideki Tohda

Yuko Hama

Chiaki Kojima

Hiromichi Kumagai

To provide a method for producing transferrin by secretion with high productivity using a transformant of, and a transformant suitable for the method. A transformant usingas a host, comprising a transferrin gene and a secretion signal peptide gene expressing transferrin to which a secretion signal peptide which functions in the host binds, located in an upstream region of the transferrin gene, and having gas2 gene endogenous to the host deleted or inactivated; and a method for producing transferrin, which comprises culturing the transformant in a liquid medium and obtaining transferrin from the liquid medium.

Transformant, and method for producing transferrin

The efficiency of production of a heterologous protein by a transformant of a yeast host is improved. A method of constructing a host for expression of a foreign gene which comprises deleting or inactivating at least one gene selected from the protease-associated genes (especially, metalloprotease genes and serine protease genes) of, a host in which the above-mentioned gene is deleted or inactivated, a transformant obtained by introducing a foreign gene into the host and a method of producing a heterologous protein using the transformant. The protease-associated gene is at least one gene selected from the group consisting of psp3 (SPAC1006.01), is sxa2 (SPAC1296.03), ppp51 (SPAC22G7.01) and ppp52 (SPBC18A7.01) or at least two genes selected from the group consisting of metalloprotease genes and serine protease genes, especially, three genes psp3 (SPAC1006.01), isp6 (SPAC4A8.04) and ppp53 (SPAP14E8.04). A host in which four genes consisting of the above three genes and ppp16 (SPBC1711.12), or five genes consisting of the above four genes and ppp22 (SPBC14C8.03) are deleted or inactivated is preferred.

Growing community of inventors

Tokyo, Japan

Alimjan Idiris