Biomerieux, Inc.

Centre National De La Recherche Scientifique

Universitéde Strasbourg

Biomerieux Sa

Cnrs

Biofire Diagnostics, LLC

Biofire Defense, LLC

Other

Gen-probe, Inc.

Université Joseph Fourier

Université De Caen Normandie

Universite Joseph Fourier Domaine

Affymetrix, Inc.

Ali Laayoun

Alain Laurent

Emmanuelle Trevisiol

Jean Lhomme

Mitsuharu Kotera

Laurent Mesta

Lionel Menou

Eloy Bernal-Mendez

David E Jones

Kirk M Ririe

Aaron D Wernerehl

Agnès Dupont-Filliard

Christopher Paul Pasko

Andrew C Hatch

Sylvain Ursuegui

Erik Wong Huynh

Carole Vachon

Eloy Bernal Mendez

Frédéric Fabis

Cecile Bourget

Arnaud Burr

Christelle Tora

Michael McClellan Becker

Charles Garrett Miyada

Kenneth A Browne

Duc Do

Alain Troesch

Matthew C Friedenberg

Fred F Hajjar

Fran&ccedil;oise Guillou-Bonnici

Eric Defranc

Antoine Hoang

Eric DeFrancq

Mitsuhara Kotera

Aloke R Banerjee

Isabelle Sothier

Raphäel Veyret

Thomas Cailly

Charles Garret Miyada

Aloke Raj Banerjee

Cécile Bourget

A method for extracting nucleic acids using suitable solid supports, for example silica-based. In particular, the method includes a step of capturing nucleic acids by placing the sample in contact with a suitable solid support, characterized in that, prior to the capture step, said method includes a step of treating the sample with at least one reagent for masking the amine or carboxylic acid functional groups of the proteins and/or polysaccharides of the sample.

Method for extracting nucleic acids

Systems, methods, and apparatuses are provided for self-contained nucleic acid preparation, amplification, and analysis.

Assay devices and methods of use thereof

The present invention concerns reagents for the reversible protection of biological molecules. It relates in particular to compounds derived from azaisatoic anhydride and their uses for the protection of biological molecules, particularly enzymes, in order to block their activity. The invention also relates to the biological molecules protected in this manner and to the methods for making use of these reagents.

Reagents for reversibly protecting biological molecules

A three-layer complex including at least one magnetic compound, at least one inorganic silicate and at least one compound having an affinity for the at least one magnetic compound and/or the at least one inorganic silicate. The three-layer complex, and associated methods, may be applicable in the field of in vitro diagnostics.

Multilayer complex, method for manufacturing said complex and use of said complex

The present invention relates to a method of functionalization of at least one ribonucleic acid (RNA) molecule, contained in a liquid sample, which includes the following steps: a) providing at least: one binding molecule consisting of an aza-isatoic anhydride or a derivative thereof, one group of interest, and one linkage joining the binding molecule to the group of interest, b) reacting the anhydride function of the binding molecule with at least one hydroxyl group carried: in position 2' of the ribose of one of the RNA nucleotides, and/or in position(s) 2&#x2032; and/or 3&#x2032; of the ribose of the nucleotide at the terminal 3&#x2032; end of the RNA, and obtaining an aza-anthranilate that joins, by means of the linkage, the RNA to the group of interest.

Methods of functionalization and reagents used in such methods using an aza-isatoic anhydride or a derivative thereof, biological molecules thus treated and kits

A method of transcriptional amplification includes: (a) obtaining a mixture by combining (i) a biological sample comprising nucleic acids, (ii) amplification primers, (iii) amplification reagents including enzymes required for amplification, and (iv) at least one polyol capable of stabilizing the enzymes required for amplification; (b) denaturing the nucleic acids by heating the mixture at a temperature above 41&#xb0; C.; and (c) transcriptionally amplifying at least one target nucleic acid at a temperature above 41&#xb0; C. when present in the mixture.

Method for transcriptional amplification of nucleic acids combining steps of different temperatures

A method of amplifying a nucleic acid of interest comprises (i) treating a biological sample chemically or enzymatically to permit conversion of one type of nucleic acid base to another type of base, (ii) purifying the treated biological sample before adding amplification primers and amplification reagents to the biological sample, (iii) adding the amplification primers and amplification reagents to the biological sample, each primer being constituted of three different types of bases and being specific to a converted nucleic acid of interest or to a nucleic acid that is complementary to the converted nucleic acid of interest, and (iv) amplifying the converted nucleic acid of interest provided that the nucleic acid of interest was present in the biological sample. In the method, amplification of contaminating nucleic acids is avoided by converting the one type of nucleic acid base to another type of base prior to adding the amplification reagents to the biological sample.

Methods and kits for avoiding amplification of contaminating nucleic acids

A process for synthesis of a labelling reagent, a process for the labelling of a biological molecule, a labelled biological molecule obtained by the process, a process for labelling and fragmentation of a single or double strand nucleic acid, a labelled nucleic acid capable of being obtained by the process, a kit for detection of a target nucleic acid containing a labelled nucleic acid, a solid support onto which is attached a reagent and a process for capture of nucleic acids.

Labelling reagents having a pyridine nucleus bearing a diazomethyl function, process for synthesis of such reagents and processes for detection of biological molecules

A nucleotide probe permitting the detection of nucleic acids and constituted of a labeled nucleotide strand having three fragments: a first fragment having a first closing sequence, a second fragment, all or part of which has a recognition sequence for the molecular recognition of the target nucleic acid and a third fragment having a second closing sequence, and at least two markers, one of the ends of the strand of the detection probe being free of any marker, in which, when the two closing sequences are hybridized together, the detection probe has a completely circular shape, the closing sequences thus maintaining the probe in a conformation that cannot be detected in the absence of the target nucleic acid.

Detection probe

The present invention relates to a method for labeling biological molecules of interest contained in a biological sample, consisting in: A method for treating a biological sample is also disclosed. Said invention is preferably used in a manual or automated method for purifying nucleic acids.

Method for labeling or treating a biological sample containing biological molecules of interest, in particular nucleic acids

The present invention relates to a labeling reagent of formula: in which:

Marking reagents bearing diazo and nitro functions, methods for the synthesis of such reagents and methods for detecting biological molecules

The present invention relates to a labeled oligonucleotide comprising a first nucleotide segment and a second nucleotide segment, complementary to a target sequence, a fluorophore, a quencher and at least one alpha-anomeric nucleoside. The invention also relates to the use of such an oligonucleotide and also to a process using such an oligonucleotide.

Labeled oligonucleotide

The present invention relates to a temperature-stable labeling reagent of formula (0): in which: The present invention also describes a method for the synthesis of said labels and also applications for the labeling of biological molecules, in particular of nucleic acids, with a labeling reagent bearing the diazomethyl function. The invention is particularly suitable for use in the field of diagnostics.

Labeling reagents, methods for the synthesis of such reagents and methods for the detection of biological molecules

The present invention relates to a temperature-stable labeling reagent of formula: in which The present invention also describes a method for synthesizing said markers as well as applications for labeling biological molecules, in particular nucleic acids, with a labeling reagent carrying the diazomethyl functional group. The invention finds a preferred application in the field of diagnosis.

Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules

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Colombe, France

Ali Laayoun