The University of Texas System

Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Institut National De La Sante Et De La Recherche Medicale

Université Grenoble Alpes

Centre Hospitalier Universitaire Grenoble Alpes

Ali Bouamrani

François Berger

Mauro Ferrari

Xuewu Liu

Jean-Paul Issartel

Alim-Louis Benabid

Adrien Mombrun

Ennio Tasciotti

Marie-Line Cosnier

Matthieu Dreyfus

David Ratel

Ye Hu

The invention relates to a device () for sampling a biological tissue comprising:

Device for sampling a biological tissue

The present invention relates to a sampling instrument adapted for taking a sample in situ and in vivo or taking a sample ex vivo of a target tissue or body structure, in a maximum quantity of less than 10g, the instrument comprising an elongated active member () with a distal operative end () having at least one retaining surface () arranged for contacting directly the target tissue or body structure, capturing and retaining molecules of the target tissue or body structure thereto.

Method for determining the molecular composition of a target tissue or another body structure, and its diagnostic applications

A new fractionation device shows desirable features for exploratory screening and biomarker discovery. The constituent MSCs may be tailored for desired pore sizes and surface properties and for the sequestration and enrichment of extremely low abundant protein and peptides in desired ranges of the mass/charge spectrum. The MSCs are effective in yielding reproducible extracts from complex biological samples as small as 10 &#x3bc;l in a time as short as 30 minutes. They are inexpensive to manufacture, and allow for scaled up production to attain the simultaneous processing of a large number of samples. The MSCs are multiplexed, label-free diagnostic tools with the potential of biological recognition moiety modification for enhanced specificity. The MSCs may store, protect and stabilize biological fluids, enabling the simplified and cost-effective collection and transportation of clinical samples. The MSC-based device may serve as a diagnostic tool to complement histopathology, imaging, and other conventional clinical techniques. The MSCs mediated identification of disease-specific protein signatures may help in the selection of personalized therapeutic combinations, in the real-time assessment of therapeutic efficacy and toxicity, and in the rational modulation of therapy based on the changes in the protein networks associated with the prognosis and the drug resistance of the disease.

Combinatorial multidomain mesoporous chips and a method for fractionation, stabilization, and storage of biomolecules

Growing community of inventors

Grenoble, France

Ali Bouamrani