Academia Sinica

Alfur F Hung

Pheidias C Wu

Jiun-Bo Chen

Tsewen Chang

Tse Wen Chang

Jiun-bo Chen

The invention pertains to methods of using C&#x3b5;mX peptides (e.g., GLAGGSAQSQRAPDRVL; SEQ ID NO:2) that can bind effectively induce immune responses to membrane-bound IgE (mIgE) expressed on the surface of human B lymphocytes.

C(epsilon)mX peptides for inducing immune responses to human mIgE on B lymphocytes

The invention pertains to the generation and utility of antibodies that can bind effectively to C&#x3b5;mX domain on membrane-bound IgE (mIgE) expressed on the surface of human B lymphocytes. The C&#x3b5;mX domain of 52 amino acid residues, located between the CH4 domain and the C-terminal membrane-anchor peptide on human membrane-bound epsilon chain, had been suggested as an antigenic site for immunological targeting of B cells expressing mIgE. Previous reported monoclonal antibodies, including a20, which bind to RADWPGPP (SEQ ID NO:1) peptide at the C-terminal of C&#x3b5;mX, have now been found to bind poorly to mIgE on human B cells. We have discovered that only monoclonal antibodies specific for certain segments, such as GLAGGSAQSQRAPDRVL (SEQ ID NO:2) and HSGQQQGLPRAAGGSVPHPR (SEQ ID NO:3), of C&#x3b5;mX can bind effectively to mIgE on human B cells and hence have the utility for targeting those B cells for the treatment of diseases mediated by IgE.

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Liouciou Township, Taiwan

Alfur F Hung