Hoechst Aktiengesellschaft

Degussa Aktiengesellschaft

Lubrizol Genetics, Inc.

Degussa - Huls Ag

Other

Alfred Puhler

Wolfgang Wohlleben

Klaus Bartsch

Georg Thierbach

Gerhard Wohner

Gunter Donn

Rudiger Marquardt

Gunter Muth

Hubert Mullner

Nicole Dusch

Inge Broer

Doris Hillemann

Renate Alijah

Eckhard Strauch

Walter Arnold

Susanne Grabley

Dieter Brauer

Reinhard Simon

Mike Farwick

Michael Leineweber

Jurgen Dohmen

Andreas Schafer

Frank Elischweski

Walter Pfefferle

Eugen Uhlmann

Mechthild Rieping

Peter Eckes

Hauke Hennecke

John M Watson

Andreas Tauch

Andreas Schaefer

Friedrich Wengenmayer

Wolfgang Wolleben

Gerhard Weber

Helmut Reilaender

Ursula Priefer

Agnes Schulte

Marcus Persicke

Susanne Schneiker-bekel

Lena Schaffert

Friedrich Kassing

Friedrich Hein

Gunther J Rieb

Petra-Sabine Kautz

Hans Sonnen

Frank Bachmann

Hans J Kutzner

Helmut Reilander

Siiri E Iismaa

J&ouml;rn Kalinowski

The present invention relates to Actinomycetales strains for the improved formation of acarbose. Provided are Actinomycetales strains which are engineered to overexpress dTDP-D-glucose-4,6-dehydratase (AcbB) and/or uridyltransferase (GtaB). Also provided are Actinomycetales strains which are engineered to have a reduced or absent expression of the small carbohydrate binding protein (Cgt) and/or a reduced or absent expression of genes which are essential for carotenoid synthesis. Also provided are tools, methods and means to generate these strains.

Methods for the improved formation of acarbose

The invention relates to a process for the preparation and improvement of D-pantothenic acid-producing microorganisms by amplification of nucleotide sequences which code for ketopantoate reductase, in particular the panE gene, individually or in combination with one another, and optionally additionally of the ilvC gene, the microorganisms containing these nucleotide sequences, and a process for the preparation of D-pantothenic acid comprising fermentation of these microorganisms, concentration of pantothenic acid in the medium or in the cells of the microorganisms, and isolation of the D-pantothenic acid.

Process for the preparation of pantothenic acid by amplification of nucleotide sequences which code for the ketopantoate reductase

Plasmids from Corynebacterium glutamicum and use thereof

The invention is a method for the production of D-pantothenic acid by the fermentation of microorganisms in which at least the panD gene is enhanced (overexpressed), optionally in combination with the panB and/or panC gene, to provide for the enrichment of the pantothenic acid in the medium or the cells of the microorganisms.

Method for the fermentative production of D-pantothenic acid by enhancement of the panD gene in microorganisms

Method for the fermentative production of D-pantothenic acid using strains of the family enterobacteriaceae

Mutant E. coli kiz strains for production of pantothenic acid

Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.

Phosphinothricin-resistance gene, and its use

Deacetylase genes for the production of phosphinothricin or phosphinothricyl-alanyl-alanine, processes for their isolation, and their use. The invention relates to deacetylase genes, to processes for their isolation, and to their use, in particular for the production of transgenic plants using tissue-specific promoters. It is possible to prevent the development of certain parts in these plants in a targeted manner. With the aid of deacetylase genes, it is furthermore possible to identify and isolate tissue-specific promoters in transgenic plants.

Deacetylase genes for the production of phosphinothricin or

The invention relates to deacetylase genes, to processes for their isolation, and to their use, in particular for the production of transgenic plants using tissue-specific promoters. It is possible to prevent the development of certain parts in these plants in a targeted manner. With the aid of deacetylase genes, it is furthermore possible to identify and isolate tissue-specific promoters in transgenic plants.

The invention relates to a transposon consisting of a fragment of the R plasmid pCxM82B from Corynebacterium xerosis DSM 5021, to mobilizable, non-self-transferrable vectors which contain this transposon, and to methods for the mutagenesis of gram-positive bacteria by means of the transfer of these vectors and the generation of auxotrophic mutants.

Transposon consisting of a fragment of the R plasmid pCxM82B, test

The present invention relates to a method of effecting the conjugative transfer of a mobilizable vector from cells of an E. coli mobilizer strain to gram-positive bacterial cells. The invention also relates to vectors suitable for use in such a method.

Method for the conjugative transfer of mobilizable vectors for E. coli

The kil-kor system from the plasmid pIJ101 can be utilized for regulated gene expression in Streptomycetes. For this, the KorA protein is either inactivated or eliminated to 'switch on' a particular gene.

Regulated gene expression in streptomycetes

Growing community of inventors

Bielefeld, Germany

Alfred Puhler