Leland Stanford Junior University

Other

Alfred Michael Spormann

Perry L McCarty

Galit Meshulam-Simon

Jochen A Müller

Bettina M Rosner

Gregory Von Abendroth

James Robert Swartz

Koshlan Mayer-Blackwell

Wing-On Ng

Marcus Emil Boyer

James Alan Stapleton

Joerg S Deutzmann

Ian Philip George Marshall

Chia-Wei Wang

A method of microbial electrosynthesis using co-cultures is disclosed. In particular, the invention relates to a method of microbial electrosynthesis utilizing a microbial strain capable of electron uptake from an electrode to produce hydrogen or formate in co-culture with a microbial production strain, such as a methanogen, acetogen, or other microorganism capable of synthesizing valuable products from carbon dioxide and hydrogen or formate.

Enhanced microbial electrosynthesis by using co-cultures

Combinations of reductive dehalogenase (rdh) genes are a distinguishing genomic feature of closely-related organohalogen-respiring bacteria. This feature can be used to deconvolute the population structure of organohalogen-respiring bacteria in complex environments and to identify relevant subpopulations, which is important for tracking interspecies dynamics needed for successful site remediation. The present disclosure encompasses embodiments of a nanoliter qPCR platform to identify organohalogen-respiring bacteria by quantitatively identifying major orthologous reductive dehalogenase gene groups.

Nanoliter qPCR platform for parallel quantitative assessment of reductive dehalogenase genes

Briefly described, embodiments of the present disclosure relate to methods and systems for identifying and classifying taxa of organohalide-respiring bacteria, including methods and systems for identify and classify two or more taxa of

Methods of identifying and classifying organohalide-respiring bacteria

Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by-like organisms. An isolated strain of bacteria,sp. strain VS, that metabolizes vinyl chloride is provided; the genetic sequence of the enzyme responsible for vinyl chloride dehalogenation; methods of assessing the capability of endogenous organisms at an environmental site to metabolize vinyl chloride; and a method of using the strains of the invention for bioremediation.

Microbial reductive dehalogenation of vinyl chloride

Functional oxygen-sensitive proteins are expressed in a heterologous diazotrophic cyanobacteria cell in the presence of ambient extracellular oxygen.

Expression of heterologous functional oxygen-sensitive proteins in filamentous cyanobacteria

Enzymatically active hydrogenase is synthesized in a cell-free reaction. The hydrogenases are synthesized in a cell-free reaction comprising a cell extract derived from microbial strains expressing at least one hydrogenase accessory protein. In some embodiments, the extracts are produced under anerobic conditions.

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Stanford, CA, United States of America

Alfred Michael Spormann