Life Technologies Corporation

Life Technologies As

Alfred Lui

Steven Menchen

Prasanna Krishnan Thwar

Geir Fonnum

Dmitriy Gremyachinskiy

Alan Blanchard

Shaheer Khan

Luisa Andruzzi

Lily Lu

Craig Stolarczyk

Synne Larsen

Guobing Xiang

Tanya Sokolsky

Wolfgang Hinz

Astrid Evenroed Molteberg

Allen Wong

Jeffrey Frediani

Pontus Lundberg

Lene Husaboe

Elisabeth Breivold

M Talha Gokmen

Talha M Gokmen

A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

Conjugated polymeric particle and method of making same

A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

Substrates and methods useful in sequencing

A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

Conjugation of carboxyl functional hydrophilic beads

A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

Polymer substrates formed from carboxy functional acrylamide

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Alfred Lui