Dh Technologies Development Pte. Ltd.

Mds Inc.

University of Manitoba

Fluidigm Canada Inc.

Applera Corporation

Mds Analytical Technologies, a Business Unit of Mds Inc.

National Research Council of Canada

Standard Biotools Canada Inc.

Dh Technologies Pte. Ltd.

Applied Biosystems (canada) Limited

Mds Inc. Doing Business Through Its Mds Sciex Division

Alexandre V Loboda

Andrew N Krutchinsky

Werner N Ens

Kenneth G Standing

Victor L Spicer

Igor V Chernushevich

Bruce A Thomson

Bruce Andrew Collings

Mircea Guna

Hassan Javaheri

Thomas R Covey

Vladimir I Baranov

Charles L Jolliffe

Bradley B Schneider

John Lawrence Campbell

Nic G Bloomfield

Yves Le Blanc

Frank Londry

Paul B Corkum

John Vandermey

William Morgan Loyd

David Rayner

Christopher Mark Lock

Gregor Sprah

Igor Chernushhevich

Andrei Krouttchinskikh

Igor Chernushevich

Erich W Ens

The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.

Sample analysis for mass cytometry

Ion optics devices and related methods of making and using the same are disclosed herein that generally involve forming a plurality of electrode structures on a single substrate. An aspect ratio of the structures relative to a plurality of recesses which separate the structures can be selected so as to substantially prevent ions passing through the finished device from contacting exposed, electrically-insulating portions of the substrate. The substrate material can be a material that is relatively inexpensive and easy to machine into complex shapes with high precision (e.g., a printed circuit board material). In some embodiments, discrete ion optical elements are disclosed which can be formed from a core material to which an electrically-conductive coating is applied, the core material being relatively inexpensive and easy to machine with high precision. The coating can be configured to substantially prevent outgassing from the core under the vacuum conditions typically experienced in a mass spectrometer.

Ion optical elements

Systems and methods are provided for correcting uniform detector saturation. In one method, a mass analyzer analyzes N extractions of an ion beam. A nonzero amplitude from an ADC detector subsystem is counted as one ion, producing a count of one for each ion of each sub-spectrum. The ADC amplitudes and counts of the N sub-spectra are summed, producing a spectrum that includes a summed ADC amplitude and a total count for each ion of the spectrum. A probability that the total count arises from single ions hitting the detector is calculated. For each ion of the spectrum where the probability exceeds a threshold value, an amplitude response is calculated, producing amplitude responses for ions found to be single ions hitting the detector. Amplitude responses are combined, producing a combined amplitude response. The total count is dynamically corrected using the combined amplitude response and the summed ADC amplitude.

Intensity correction for TOF data acquisition

An ion lens for reducing contaminant effects in an ion guide of a mass spectrometer is provided. The ion lens comprises a structural member comprising an orifice of a given radius, the structural member for supporting the ion lens at an exit region of the ion guide. The ion lens further comprises a conical member extending from the structural member, the conical member being hollow and comprising a given cone angle, and a base of the given radius, a perimeter of the base connected to a perimeter of the orifice. The conical member further comprises an aperture through an apex of the conical member, the aperture for receiving ions there through from the ion guide.

Ion lens for reducing contaminant effects in an ion guide of a mass spectrometer

In a first location of a mass spectrometer, a plurality of ionized molecules of an ion source are selected that have mass-to-charge ratios within a mass-to-charge ratio window width. The plurality of selected ionized molecules are transmitted from a first to a second location. Reagent ions are transmitted to the second location to reduce a charge state of one or more of the plurality of selected ionized molecules. A mass analyzer is used to analyze the plurality of reduced ionized molecules and produce a mass spectrum. A compound is identified from a peak of the spectrum that has a mass-to-charge ratio less than or equal to the highest mass-to-charge ratio in the window width if the noise is multiply charged and greater than the highest mass-to-charge ratio in the window width if the noise is singly charged.

Windowing combined with ion-ion reactions for chemical noise elimination

The present disclosure generally provides ionization methods and devices for use in mass spectrometry. In some embodiments, the ionization methods and devices employ short laser pulses (e.g., pulses having pulsewidths in a range of about 2 fs to about 1 ps) at a high intensity (e.g., an intensity in a range of about 1 TW/cmto about 1000 TW/cm) to ionize an analyte an ambient pressure greater than about 10Torr (e.g., an ambient pressure in a range of about 1 atmosphere to about 100 atmospheres).

Ionization with femtosecond lasers at elevated pressure

An ion source and an ion guide chamber are provided. The ion guide chamber having a gas flow, the gas flow having a longitudinal velocity and a transverse velocity. The ion guide chamber having an exit aperture and at least one ion guide. The at least one ion guide having an entrance end and an exit end with an exit cross-section wherein the exit cross-section is sized to be smaller in area than the entrance cross-section. The at least one ion guide having a plurality of elongated electrodes wherein a gap between the elongated electrodes and the shape of the elongated electrodes in the vicinity of the gap are essentially the same along the length of the at least one ion guide for confining the ions in the vicinity of the gap by a combination of the transverse velocity of the gas and the RF voltage.

Method and apparatus for improved sensitivity in a mass spectrometer

An apparatus and method for coupling RF and AC signals to provide power to a multipole in a mass spectrometer is provided. A first circuit comprises: an RF power source for providing difference mode power to the multipole via the RF signal; at least one inductor for forming at least one resonant LC circuit with the multipole for providing voltage gain for the RF signal; and a transformer, comprising a secondary tap, the transformer connected in parallel to the RF power source and further connected to the at least one inductor such that the signals injected into the secondary tap are communicated to the at least one resonant LC circuit. A second circuit comprises: an AC power source for providing common mode power to the multipole via the AC signal, an output from the second circuit connected to the secondary tap such that the common mode power is injected into the first circuit and the multipole can be operated in common mode and difference mode simultaneously; and at least one inductor for forming a resonant LC circuit with the multipole via the transformer, to provide voltage gain for the AC signal.

Apparatus and method for coupling RF and AC signals to provide power to a multipole in a mass spectrometer

Dual TDC-ADC detection systems for time of flight mass spectrometry are disclosed herein. Detection systems based upon TDC generally provide higher timing resolution as opposed to detection systems based upon ADC. However, ADC generally provides increased dynamic range over TDC. By combining TDC and ADC into a tandem detector, and adjusting performance parameters of the respective converter types, the dynamic range of the dual TDC-ADC detection can be extended beyond what either detector type could have achieved individually. Composite time of flight mass spectra can be generated by aggregating individual mass spectra acquired from multiple time-of-flight extractions, and selecting the number of time-of-flight extractions to ensure overlap between the ADC and TDC dynamic ranges in the dual TDC-ADC detector system.

Method and system for operating a time of flight mass spectrometer detection system

Systems, methods and apparatus for radial amplitude assisted transfer (RAAT) in mass spectrometers are provided in which ions for RAAT are accelerated along a longitudinal axis of a mass spectrometer in order to decrease the magnitude of excitation energy of radially excited ions in an ion trap that allows the radially excited ions to exit the ion trap. Hence, the radially excited ions exit the ion trap with reduced radial energy thereby decreasing the exit angle of the radially exited ions from the ion trap. Furthermore, combined forces on the ions are such that radially excited ions exit the ion trap while unexcited ions remain in the ion trap.

Linear ion trap for radial amplitude assisted transfer

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Winnipeg, Canada

Alexandre V Loboda