Visible Genetics Inc.

Angle Europe Limited

Siemens Healthcare Diagnostics Gmbh

Bayer Healthcare LLC

Georgia-pacific Consumer Products LP

Smart Wave Technologies Corporation

Axela Inc.

Siemens Medical Solutions Diagnostics

Alexandre M Izmailov

Paul Waterhouse

Paul D Smith

Henryk Zaleski

Brian Pak

Tomoyuki Sen

John K Stevens

Marina T Larson

Bruno C Maruzzo

Thomas D Yager

Guido Hennig

John A Renfrew

Thomas Yager

David Englert

Thomas J Digby

Heike Euting

Murugathas Yuwaraj

Kang Chang Yeh

Paul James Ruthven

James M Dunn

Daniel James Geddes

Harold J Goeking

Peter Zosimadis

James Leushner

Panagiotis Zosimadis

James W Cassidy

Paul Timothy Smith

Stephen Darasch

Guido Henning

The present disclosure provides a method of cell sedimentation and retention of target cells, for example circulating tumour cells, CTC, from a fluid sample onto a solid support. The method comprises placing a fluid medium comprising the target cells in a fluid chamber, the fluid chamber having an open end sealed against a surface of the solid support. The method further comprises subjecting the fluid medium to centrifugation within the fluid chamber to induce sedimentation of the target cells and promote cell adhesion to the surface of the solid support. The method further comprises, post-centrifugation, positioning a fluid absorbing element in the fluid chamber to remove fluid from the fluid chamber. The method further comprises controlling a flow rate of the fluid being absorbed by the fluid absorbing element such that the sedimented cells are not detached from the surface of the solid support.

Cell recovery method and device

The present disclosure provides a cell harvesting method and device for the efficient sedimentation and retention of cells from liquid samples onto a solid support with low cell losses and low impact on cell morphology. The device has two configurations, one being for use in a centrifuge to centrifuge the sample, a second for controlled release of the liquid post centrifugation. The device includes a base to hold a solid support that receives cells on a top surface thereof that releasably holds a fluid chamber which has a first opening with a gasket surrounding the opening with that bears against a top surface of the support. An opening receives the liquid sample and the gasket defines an area into which the targeted cells deposit. A first cap closes the second opening during centrifugation. Post centrifugation the first cap is removed and a fluid absorbing element and cap, designed to provide controlled removal of the liquid and to prevent positioning of a tip of the absorbing element to a distance from the solid support shorter than a predefined distance is inserted into the fluid chamber.

The present disclosure provides a cell harvesting method for the efficient sedimentation and retention of cells from liquid samples onto a solid support with low cell losses and low impact on cell morphology.

The present disclosure provides a cartridge and method to move fluids within the cartridge that simplifies the design and removes the need for any internal valves or metering devices. The design is amenable to injection molded manufacturing lowering cost for large volume manufacturing. The design can be adapted to carry out both sample preparation and detection of biological substances including nucleic acids and proteins.

Disposable bioassay cartridge and method of performing multiple assay steps and fluid transfer within the cartridge

The present disclosure provides a diffraction based biosensor containing at least two diffraction gratings. The first grating is referred to as an in-coupling diffraction grating and the coherent light source (laser) is directed to illuminate the in-coupling grating, and the biosensor is configured such that a selected order of the light beam diffracted from the in-coupling diffraction grating illuminates a second biosensor grating coated with analyte-specific receptors which are selected to preferentially bind with analytes being tested for that may or may not be located in a sample being tested.

Diffraction based biosensor containing two diffractive gratings

Systems and methods for differentiating the spectral response of various optical coatings between a transmitter and receiver. The system is effective in determining if an optical coating produces an authorized spectral response for determining if a product having that optical coating is authorized to be used with another product.

Signal and detection system for keying applications

The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

Method for purification of nucleic acids, particularly from fixed tissue

A method of dispensing product includes determining by a processor if product loaded into a dispenser is authorized for use in the dispenser by identifying a reference indication associated with the product; in response to determination that the product is authorized, dispense a first amount of product; and in response to determination that the sheet product is unauthorized, dispense a second amount of sheet product, wherein the second amount of sheet product is different than the first amount. To determine if the sheet product is authorized, a pigment is excited to emit a light with an intensity signature. A portion of the intensity signature is measured and compared to a desired value. If the portion is substantially equal to the desired value, the sheet product is authenticated.

Product, dispenser and method of dispensing product

The present invention relates to methods for resolving convoluted peaks in a chromatogram into one or more constituent peaks using peak resolution values. The peaks methods of the invention determine empirical peak resolution values of 'well-defined' or 'isolated' peaks in the data, then extrapolate these empirical resolution values to peaks in neighboring regions to predict the number of constituent peaks at a given peak position. Predicted peak resolution values are compared to observed peak resolution values of low-resolution or convoluted peaks to determine the number of constituent peaks in the convoluted peaks. These methods enable extension of the region of data that can used for identifying nucleotide sequences, and increase base-calling accuracy in the low-resolution region (end region) of data.

Methods for resolving convoluted peaks in a chromatogram

The present invention is directed to a method for alignment of nucleic acid data traces. The method involves selecting reference alignment points from among internal peaks representing highly conserved bases, preferably consisting of heterogeneous multiplets. The alignment points may also optionally include the primer peak and/or the full-length peak. Reference position numbers are assigned to these alignment points reflecting the known relative position of the alignment point, a sequence position number is assigned to peaks in the data traces so as to maximize assigning the sequence position number and the reference position number to the same base. Optionally, the method may include the step of determining the average peak spacing interval between alignment points and assigning a sequence position number to peaks occurring at the intervals. The data traces are then aligned based on the assigned sequence position numbers.

Method and apparatus for alignment of DNA sequencing data traces

A simulated electrophoretic trace is prepared by first obtaining an input file containing an input base sequence comprising a string of letters (A, C, G and/or T) in an order corresponding to the input base sequence, and then modifying the input file using one or more functions to take into account perturbations associated with (1) changes in peak intensity as a function of base number; (2) peak shape as a function of base number; (3) peak skew; (4) spacing between peaks; (5) background; (6) noise; (7) spectral cross-talk; (8) instrumental effects and/or (9) gel electrophoresis effects to produce a modified file representing a simulated electrophoretic trace. The method may be performed using a specially adapted apparatus.

Electrophoretic trace simulator

In order to align DNA sequence data traces, an experimental data trace representing the positions of a first species of base within a target polynucleotide and a reference data trace representing the positions of a second species of base (which may be the same as or different from the first species) within a reference polynucleotide are obtained by separating appropriate sequencing fragments generated from the target and reference polynucleotides on an electrophoresis gel. For each reference data trace, a plurality of peaks corresponding to fragments having a size in the range of 40 to 1200 bases are selected. A base number is assigned to each of the selected peaks in the reference data trace, and a numerical &ldquo;peak file&rdquo; is created with information about the peak number and migration time (or distance). This peak file is analyzed to determine a set of polynomial coefficients which will allow substantial linearization of a plot of peak number versus separation between adjacent peaks and alignment of the traces with respect to each other. These coefficients are used to create a corrected time scale identifying where peaks should be located on a given experimental gel. This corrected time scale is used to guide the sampling of the experimental data, and for assignment of peaks within the data.

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Toronto, Canada

Alexandre M Izmailov