Mauna Kea Technologies

Centre National De La Recherche Scientifique

Université De Limoges

Alexandre Loiseau

Bertrand Viellerobe

Magalie Genet

Benjamin Abrat

Alain Barthelemy

Francois Lacombe

Dominique Pagnoux

François Lacombe

Anne Osdoit

Geneviève Bourg-Heckly

Frédéric Louradour

Frederic Berier

Charlotte Cave

Mickael Lelek

Florence Jean

Sandrine Vilette

Michaël Lelek

A system for carrying out fibered multiphoton microscopic imagery of a sample () for use in endoscopy or fluorescence microscopy includes: a femtosecond pulsed laser () for generating a multiphoton excitation laser radiation; an image guide () having a number of optical fibers and permitting the sample to be illuminated by a point-by-point scanning in a subsurface plane; pre-compensating elements () for pre-compensating for dispersion effects of the excitation pulses in the image guide (), these elements being situated between the pulsed laser and the image guide (); scanning elements for directing, in succession, the excitation laser beam in a fiber of the image guide, and; in particular, an optical head () for focussing the excitation laser beam exiting the image guide in the sample ().

System and method for carrying out fibre-type multiphoton microscopic imaging of a sample

The invention relates to a method for producing a fluorescent fiber image of a sample, wherein a sample is scanned with the aid of an excitation signal; the fluorescent signal emanating from the sample is detected, wherein the excitation signal and fluorescent signal use the same optical path; the optical path is used to excite at least two fluorophores contained in the sample; a final image is produced, including areas that are colored according to the at least two fluorophores. The multimarking according to the invention makes it possible to simultaneously acquire two images in two different wavelength bands. The system according to the invention can comprise a spectrometer for spectral quantification of the fluorescent signal.

Multimarking fibre-type fluorescence microscopic imaging method and system

An equipment includes an excitation source (), elements for injecting () an excitation signal produced by the source in an ordered bundle () of flexible optical fibers, elements for analyzing () an emitted autofluorescence signal. The equipment also includes at the output of the optical fiber bundle () an optical head () designed to be placed in contact with the biological tissue (), the optical head being equipped with optical elements adapted to cause the excitation signal output from the bundle () to converge into a subsurface analyzing zone (), the same optical fiber(s) used for excitation of the bundle () being used for detecting the signal emitted by the subsurface analyzing zone, elements (D) placed upstream of the injection elements () being further provided to separate the wavelength of the excitation signal and the wavelength of the autofluorescence signal.

Equipment for subsurface autofluorescence spectroscopy

An optical head for equipping the distal end of a flexible optical fiber bundle, designed to be urged into contact with an analyzing surface and including optical elements for focusing an excitation signal into a so-called excitation focal point located at a specific depth beneath the analyzing surface and for sampling a signal backscattered by the excitation focal point which is carried back by the fiber bundle. The head includes an optics-holder tube wherein are inserted on one side the distal end portion of the fiber bundle and on the other optical elements, the latter including a plate placed in contact with the end of the fiber bundle whereof the index is close to that of the fiber core and a focusing optical block, an output window being further provided adapted to provide index adaptation so as to eliminate parasitic reflection occurring on the analyzing surface.

Miniaturized focusing optical head in particular for endoscope

Growing community of inventors

Paris, France

Alexandre Loiseau