Trilink Biotechnologies, Inc.

Hogrefe, Richard I.

Lebedev, Alexandre

Woolf, Tod M.

Alexandre Lebedev

Tod M Woolf

Richard I Hogrefe

Dongwon Shin

Anton P McCaffrey

Gerald Zon

Natasha Paul

Jonathan Shum

Sabrina Shore

Inna Koukhareva

Anton P Mccaffrey

Provided herein are methods and compositions for synthesizing 5'Capped RNAs wherein the initiating capped oligonucleotide primers have the general formGppp[N][N]whereinG is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, 'n' can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.

Compositions and methods for synthesizing 5'-capped RNAs

Provided herein are methods and compositions for synthesizing 5′Capped RNAs wherein the initiating capped oligonucleotide primers have the general formGppp[N][N]whereinG is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, 'n' can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.

Compositions and methods for synthesizing 5′-Capped RNAs

Provided herein are methods and compositions for synthesizing 5'Capped RNAs wherein the initiating capped oligonucleotide primers have the general formGppp[N][N]whereinG is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, 'n' can be any integer from 0 to 4 and 'm' can be an integer from 1 to 9.

Compositions and methods for synthesizing 5'-Capped RNAs

Compositions and methods for synthesizing 5'-Capped RNAS

Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template. In addition, methods are applied to the preparation of nucleic acid libraries using modified acceptor probes and modified donor probes that reduce or eliminate probe dimerization during the ligation process.

Chemically modified ligase cofactors, donors and acceptors

Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template.

The present invention provides methods and compositions for nucleic acid amplification. These methods involve the use of oligonucleotide primers in temperature dependent nucleic acid amplification reactions. In certain aspects, the methods are accomplished by use of certain modified oligonucleotide primers which provide utility in nucleic acid amplification. In preferred embodiments, the oligonucleotide primers are modified with particular chemical groups such as esters.

Phosphotriester-modified oligonucleotide primers for nucleic acid amplification

Provided herein are methods and compositions for nucleic acid replication. These methods involve the use of 3'-substituted nucleoside 5&#x2032;-triphosphates or 3&#x2032;-substituted terminated primers in nucleic acid replication reactions. In certain aspects, the methods are accomplished by use of 3&#x2032;-substituted NTPs and/or 3&#x2032;-substituted terminated primers which provide utility in nucleic acid replication. In preferred embodiments, the NTPs and/or primers are substituted at the 3&#x2032;-position with particular heat labile chemical groups such as ethers, esters or carbonate esters.

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San Diego, CA, United States of America

Alexandre Lebedev