Horiba Abx Sas

Horiba Abx Sas / Horiba Ltd

Alexandra Urankar

Philippe Nerin

Benoit Merchez

Gilles Cauet

Didier Cremien

Sebastien Raimbault

Moustapha Hariche

Benoît Merchez

Sébastien Raimbault

The present invention relates to a method for compensating for the breakdown of a reagent stored in an aqueous phase comprising at least one fluorescent compound and enabling the identification of particles, including the steps of: (i) measuring the fluorescence level FLUOm(t) of particles marked with said reagent; (ii) measuring the absorbance at at least one wavelength of a solution of said reagent, at a time t close to the time of said fluorescence level FLUOm(t) measurements, so as to determine at least one current optical density DO(t) of the reagent; and (iii) calculating a correction of the fluorescent level measurements using said at least one current optical density DO(t) and at least one initial optical density DO(0) of the reagent that has not been broken down. The invention also relates to a biological analysis device implementing the method.

Method for compensating for the aging of a reagent during fluorescence measurements carried out on particles, and biological analysis device implementing the method

The invention relates to an electro-optical device for measurements of flow for the characterization of microparticles, comprising a measurement chamber (CUM) in which there circulates the flow of the fluid to be characterized, at least two luminous sources (S,S) of disjoint spectra, a device for measuring resistivity (RES), and at least three other detectors (D,D,D) each allowing the measurement of an optical parameter, the optical parameters being chosen from among fluorescence (FL), extinction (EXT), wide angle diffraction (SSC) and small angle diffraction (FSC).

Device and method for multiparametric measurements of microparticles in a fluid

The invention relates to a method of classifying and flow measuring the refringence of at least two populations of particles present in a fluid. The method uses a light source that has small coherence time, with a coherence length Lc&#x3c;100 &#x3bc;m, that is used under extinction conditions at a center wavelength selected as a function of a range of volumes and of a range of refractive indices expected for the particles under consideration. The method uses a device that, together with the light source, forms a converging illuminating beam of aperture angle that is selected as a function of the range of volumes and the range of refractive indices expected for the particles under consideration at the selected center wavelength.

Device and method for multiparameter measurements of microparticles in a fluid

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Saint Clement de Riviere, France

Alexandra Urankar