Roche Sequencing Solutions, Inc.

Integrated Dna Technologies, Inc.

Kapa Biosystems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Alexandra Hui Wang

Jenny A Johnson

Martin Ranik

Rui Chen

Ashley Hayes

Severine Margeridon

Daleen Badenhorst

Persis Wadia

Aruna Arcot

Toummy Guettouche

Katharine Dilger

Rachael Cunningham

The present disclosure provides a method of preparing a library of nucleic acids having modular end sequences. The method includes combining a pool of different modular nucleic acid tags with a nucleic acid sample, the nucleic acid sample including a plurality of double-stranded target nucleic acids. The method further includes joining the ends of each of the double-stranded target nucleic acids to tags selected from the pool of different modular nucleic acid tags to form a plurality of doubly-tagged target nucleic acids, amplifying each of the doubly-tagged target nucleic acids, thereby preparing a library of nucleic acids having modular end sequences, and detecting the library of amplified nucleic acids having modular end sequences.

System and method for modular and combinatorial nucleic acid sample preparation for sequencing

Described herein are methods and compositions for improved hybridization capture for enriching for target nucleic acid sequences from a population of nucleic acids. In one aspect, the methods and compositions are useful for Next Generation Sequencing (NGS) applications. The methods and compositions include combining in solution capture probes and target nucleic acid and permitting hybridization of the target nucleic acid to the capture probes under conditions to promote efficient hybridization. Following hybridization, the probe/target complex is immobilized to a capture material while simultaneously incubating at an optimum hybridization temperature. The incubation denatures unwanted nucleic acids from the capture probes further enriching for target nucleic acid.

Hybridization capture methods and compositions

The invention is a novel method of separately sequencing each strand of a nucleic acid involving the use of an adaptor comprising a strand cleavage site or a strand synthesis termination site. The adaptor may also be self-priming at the strand cleavage site.

Generation of single-stranded circular DNA templates for single molecule sequencing

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Mountain View, CA, United States of America

Alexandra Hui Wang