Algenol Biotech LLC

Algenol Biofuels, Inc.

Alexandra Friedrich

Heike Enke

Dan Kramer

Kerstin Baier

Ulf Dühring

Frank Uliczka

Irina Piven

Kui Wang

Tuo Shi

Masami Inaba

Ulf Duehring

Ulf DÜHRING

A genetically enhanced cyanobacterial host cell,sp. ABICyanoI, is disclosed. The enhancedsp. ABICyanoI produces a compound or compounds of interest.

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This invention provides a method for the prolonged production of ethanol using a metabolically enhanced cyanobacterium.

Metabolically enhanced cyanobacterium with sequentially inducible production genes for the production of a first chemical compound

One embodiment of the invention provides a genetically enhancedsp. host cell comprising at least one first recombinant gene encoding a first protein for the production of ethanol under the transcriptional control of a first inducible promoter, having at least 85%, 90% or 95% sequence identity to an endogenous inducible promoter of thesp. host cell.

This invention provides a metabolically enhanced cyanobacterium for the production of a chemical compound of interest, having at least two first production genes encoding first biocatalysts for the production of the first chemical compound. One of the two first production genes is under the transcriptional control of a first promoter for the first production gene, whereas the other of the two first production genes is under the transcriptional control of a second promoter for the first production gene. The first promoter and second promoter are separately inducible under different conditions and the at least two first biocatalysts catalyze the same chemical reaction. Metabolically enhanced cyanobacteria according to the present invention allow prolonged production of first chemical compounds. Further provisions of the present invention include, inter alia, a method for producing the metabolically enhanced cyanobacterium and a method for producing a first chemical compound by culturing the metabolically enhanced cyanobacterium.

A cyanobacterial host cell,sp., that harbors at least one recombinant gene for the production of a chemical compounds is provided, as well as vectors derived from an endogenous plasmid isolated from the cell.

A genetically enhanced cyanobacterial host cell,sp. ABICyano1, is disclosed. The enhancedsp. ABICyano1 produces a compound or compounds of interest.

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Berlin, Germany

Alexandra Friedrich