Carl Zeiss Microscopy Gmbh

Ruprecht-Karls-Universitaet Heidelberg

Alexandra F Elli

Martin Edelmann

Andreas Schertel

Rasmus Schroeder

Methods and devices for magnified imaging of three-dimensional samples are disclosed. The methods include imaging sample sections of a sample section series using a first particle-optical device. Coordinates of the imaged sample point are acquired and stored in such a way that the coordinates of the imaged sample point can be associated with the respective image of this sample point. The method also includes selecting a volume of interest (VOI), and transmitting the coordinates of the selected VOI to a second particle-optical device. In addition, the method includes imaging the selected VOI by means of the second particle-optical device. A plurality of planes of a sample section are imaged in order to obtain a 3D image of the selected VOI.

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Oberkochen, Germany

Alexandra F Elli