Verenium Corporation

Bp Corporation North America Inc.

Basf Enzymes LLC

Alexander Varvak

Shaun Healey

David Paul Weiner

Mark Joseph Burk

Toby Howard Richardson

Grace DeSantis

Peter Luginbuhl

Ellen Burke

Connie Jo Hansen

Geoff Hazlewood

William H Greenberg

Justin Stege

Kelvin Wong

David Blum

Thomas Todaro

Kristine Chang

Charles Hancock

Thomas Woodward

Mircea Podar

Chris S Lyon

John Poland

David B Weiner

Hwai Wen Chang

Scott Beaver

Scott W Beaver

Hwai Chang

Grace Desantis

In alternative embodiments, the invention provides polypeptides having a lignocellulolytic (lignocellulosic) activity, e.g., a ligninolytic and cellulolytic activity, including, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase (cbhl) (e.g., an exo-cellobiohydrolase, e.g., having an 'exo' activity that can processively release cellobiose units &#x3b2;-1,4 glucose-glucose disaccharide), a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a (&#x3b2;-xylosidase) and/or an arabinofuranosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides and nucleic acids of the invention are used in a variety of pharmaceutical, agricultural and industrial contexts; for example, as enzymes for the bioconversion of a biomass, e.g., lignocellulosic residues, into fermentable sugars, where in one aspect these sugars are used as a chemical feedstock for the production of ethanol and fuels, e.g., biofuels, e.g., synthetic liquid or gas fuels, including ethanol, methanol and the like.

Variant CBH I polypeptides

This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.

Lyase enzymes, nucleic acids encoding them and methods for making and using them

The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal &#x3b2;-1,4-xylosidic linkages or endo-&#x3b2;-1,4-ghicanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.

Xylanases, nucleic acids encoding them and methods for making and using them

The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides.

Variant CBH I polypeptides with reduced product inhibition

This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. X&#x2550;NO2, Cl, Br, NH2, OH, H, alkyl at one or several o, m, and p positions R&#x2550;H or alkyl.

The invention provides aldolases, nucleic acids encoding them and methods for making and using them, including chemoenzymatic processes for making &#x3b2;,&#x3b4;-dihydroxyheptanoic acid side chains and compositions comprising these side chains, e.g., [R&#x2014;(R*,R*)]-2-(4-fluorophenyl)-b,d-dihydroxy-5-(1-methylethyl)-3-phenyl-4-(phenylamino)-carbonyl]-1H-pyrrole-1-heptanoic acid (atorvastatin, LIPITOR&#x2122;), rosuvastatin (CRESTOR&#x2122;), fluvastatin (LESCOL&#x2122;), related compounds and their intermediates.

Chemoenzymatic methods for the synthesis of statins and statin intermediates

The invention provides novel aldolases, nucleic acids encoding them and methods for making and using them, including chemoenzymatic processes for making &#x3b2;,&#x3b4;-dihydroxyheptanoic acid side chains and compositions comprising these side chains, e.g., [R-(R*,R*)]-2-(4-fluorophenyl)-b,d-dihydroxy-5-(1-methylethyl)-3-phenyl-4-(phenylamino)-carbonyl]-1H-pyrrole-1-heptanoic acid (atorvastatin, LIPITOR&#x2122;), rosuvastatin (CRESTOR&#x2122;), fluvastatin (LESCOL&#x2122;), related compounds and their intermediates.

Aldolases, nucleic acids encoding them and methods for making and using them

Growing community of inventors

San Diego, CA, United States of America

Alexander Varvak