Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.

Danziger Innovations Ltd.

Alexander Vainstein

Amir Zuker

Marianna Ovadis

Ira Marton

Hanan Itzhaki

Elena Marhevka

Yael Leitner-Dagan

Michal Moyal-Ben-Zvi

Michael Vishnevetsky

Ben Rimon-Spitzer

Arik Honig

Elena Shklarman

A nucleic acid construct is provided. The construct comprises a tobacco rattle virus (TRV) sequence and a nucleic acid sequence encoding a single guide RNA (sgRNA) that mediates sequence-specific cleavage in a target sequence of a genome of interest, wherein the TRV sequence is devoid of a functional 2b sequence. Also provided are plant cells comprising the construct and uses of the construct in gene editing.

Nucleic acid constructs for genome editing

A method of generating genotypic variation in a genome of a plant is disclosed. The method comprising introducing into a gamete or a gamete producing tissue of the plant at least one viral expression vector encoding at least one chimeric nuclease which comprises a DNA binding domain, a nuclease and a localization signal to a DNA-containing organelle, wherein the DNA binding domain mediates specific targeting of the nuclease to the genome of the plant, wherein the introducing is performed such that the gamete or gamete producing tissue expresses the chimeric nuclease but not all plant tissues express the chimeric nuclease, thereby generating genotypic variation in the genome of the plant.

Generating genotypic variations in plant genomes by gamete infection

A method of generating genotypic variation in a genome of a plant is disclosed. The method comprising introducing into the plant at least one viral expression vector encoding at least one chimeric nuclease which comprises a DNA binding domain, a nuclease and a localization signal to a DNA-containing organelle, wherein the DNA binding domain mediates specific targeting of the nuclease to the genome of the plant, thereby generating genotypic variation in the genome of the plant.

Plant viral expression vectors and use of same for generating genotypic variations in plant genomes

A method of enhancing production of a phenylpropanoid compound in a plant or plant cell is disclosed. The method comprising: (a) expressing in the plant or plant cell a heterologous polynucleotide which produces a myb gene of the phenylpropanoid pathway; and (b) contacting the plant or plant cell with at least one substrate of the phenylpropanoid pathway, the substrate being upstream to the production of the phenylpropanoid compound, thereby enhancing the production of a phenylpropanoid compound in the plant or plant cell.

Methods of modulating production of phenylpropanoid compounds in plants

Methods of increasing tolerance of a plant to stress are provided. According to an exemplary aspect a method of increasing tolerance of a plant to a biotic stress is provided. The method comprising expressing within the plant an exogenous fibrillin/CDSP34 thereby increasing the tolerance of the plant to the biotic stress. Also provided are nucleic acid constructs and transgenic plants comprising same.

Transgenic plants exhibiting increased tolerance to stress and methods of generating same

A method of transforming a carnation (L.) plant genome with a DNA molecule. The method comprises (a) preparing stem explants from carnation cuttings; (b) wounding the explants by microprojectile bombardment; (c) cocultivating the wounded explants withcomprising the DNA molecule under defined conditions of exposure to dark followed by light; (d) excising shoots from the cultivated wounded explants and removing the leaves from the shoots; and (e) culturing the leaves to obtain transgenic shoots transformed with the DNA molecule. Also disclosed are a rolC-transgenic carnation with improved agronomic traits and enhancement of flower fragrance by antisense suppression of the flavonoid gene fht.

Transgenic plants and method for transforming carnations

A method for production of high amounts of carotenoids in plants, bacteria or cells. Carotenoid-associated proteins CHRC and CHRD isolated from corollas of , chrc gene, CHRC deduced sequence, the molecular cloning, cDNA and RNA. A new CHRC promoter able to direct expression for foreign genes. The cloned promoter comprising approximately 3.5 Kb of the CHRC upstream region.

Growing community of inventors

Rechovot, Israel

Alexander Vainstein