Merck + Co., Inc.

Alexander U Bertland, Ii

George P Lampson

William J Miller

Eugene B Buynak

Alfred A Tytell

Hepatitis B PreS2+S antigen is isolated from plasma by adsorption on an affinity chromatography column, elution with a chaotropic agent and treatment with concentrated urea at an elevated temperature. The process retains all or substantially all of the preS2+S antigen.

Pres2+S hepatitis B vaccine derived from plasma

A herpes simplex type I subunit vaccine is prepared from infected chick embryo cells by ultra extraction of the virus while the infected cells are still attached to the growth surface.

Herpes simplex type I subunit vaccine

A herpes simplex type 1 subunit vaccine is prepared from infected chick embryo cells by urea-extraction of the virus while the infected cells are still attached to the growth surface.

Herpes simplex type 1 subunit vaccine

Hepatitis B antigen useful as vaccine is prepared from clarified plasma of hepatitis B donors by treating with pepsin at a pH wherein pepsin is enzymatically active, treating with urea, and optionally treating with formaldehyde.

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Lansdale, PA, United States of America

Alexander U Bertland, Ii