Carlsberg A/s

Alexander Striebeck

Ole Olsen

Søren Knudsen

Birgitte Skadhauge

Toni Wendt

Hanne Cecille Thomsen

Magnus Wohlfahrt Rasmussen

Massimiliano Carciofi

Rasmussen Magnus Wohlfahrt

In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms.

Method to screen for a mutant within a population of organisms by applying a pooling and splitting approach

Growing community of inventors

Frederiksberg C, Denmark

Alexander Striebeck