Global Life Sciences Solutions Germany Gmbh

General Electric Company

Global Life Sciences Solutions Operations UK Ltd

Whatman Gmbh

Qiagen Healthcare Biotechnologies Systems Gmbh

Alexander Schenk

Suzana Kiel

Peter James Tatnell

Jeffrey Kenneth Horton

Wei Sun

Klaus Hochleitner

Marcel Thieme

John Richard Nelson

Michael John Gerdes

Ralf Lenigk

Alan Stuart Pierce

Patrick McCoy Spooner

Rebecca Ngaire Fullerton

Wolfgang Thamm

Daniela Katarzynski

Georg Peter Danisch

Patrick Mccoy Spooner

The present invention relates to a device and method for sample preparation and collection. More closely the invention relates to a device to isolate DNA, RNA and proteins or other biomolecules in one single step from the same undivided sample.

Device and method for sample isolation

In a lateral flow test (LFT) device, the liquid conduit elementis formed from a substrateorhaving at least two layers, including a first layerornot exceeding 75 &#x3bc;m in thickness formed from a porous material for wicking liquid, and a second layerorof additional thickness formed from a generally non-porous polymer material acting as backing layer. The layered arrangement is incorporated into an LFT device to reduce sample volume requirements.

Lateral flow testing

Disclosed is a method and apparatus for manufacturing a continuous web of polymeric membrane and for continuous downstream processing of said web. The apparatus () comprises: a casting station () for casting the continuous web (M); a carrier () for carrying the web downstream; a membrane drier () downstream of the carrier, for drying the web; and a brushing station () downstream of the drier for brushing the web. Said drier is located immediately downstream of the carrier, and upstream of said brushing station. The apparatus () further includes an additional drying station () downstream of the brushing station (). Brushing after drying retains more surfactant in the membrane which is useful for certain applications. In addition, initial drying eliminates virtually all solvents from the membrane, but leaves some non-solvent (e.g. water) within it, which in turn fixes the surfactant on the nitrocellulose fibers, which improves significantly the consistency and reproducibility of the membrane.

Polymeric membranes

The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method includes, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.

Methods and kits for capturing sperm nucleic acids

Growing community of inventors

Dassel, Germany

Alexander Schenk