Roche Molecular Systems, Inc.

Alexander Nierth

Dieter Heindl

Stephen Gordon Will

Amar Gupta

Igor Kozlov

Hannes Kuchelmeister

Christian Wellner

Concordio Anacleto

The present invention relates to compositions and methods (reagents and protocols) for the post-synthetic modification of nucleic acids obtained from solid-phase oligonucleotide synthesis with a label (such as fluorescent dyes). The coupling reagent is the triazine-based salt 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMT-MM) in the presence of a counteranion.

Reagents and methods for post-synthetic modification of nucleic acids

The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.

Photocleavable nucleotide reagents with high stability

Catalysts act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting catalysts to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization.

Catalysts for reversing formaldehyde adducts and crosslinks

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Dublin, CA, United States of America

Alexander Nierth