Roche Molecular Systems, Inc.

Alexander Meier

Chris Steinert

Michael Zeder

A microfluidic device for thermocycling of a reaction mixture is provided. The device comprises an inlet opening, an outlet opening, a flow channel connecting the inlet opening and the outlet opening and defining a flow direction from the inlet opening through the flow channel to the outlet opening, wherein the flow channel comprises a first flow channel surface and a second flow channel surface opposite to the first flow channel surface, and an array of wells provided in the first flow channel surface for fluidic communication with the inlet opening and the outlet opening. Further, the first flow channel surface provides a first hydrophilicity and at least a part of the second flow channel surface provides a second hydrophilicity, wherein the first hydrophilicity is greater than the second hydrophilicity.

Microfluidic device

The present disclosure provides a microfluidic device () comprising at least one flow channel () connecting an inlet opening () with an outlet opening (), and an array of wells () in fluid communication with the flow channel (), wherein the flow channel () comprises an inlet portion () at the inlet opening (), an outlet portion () at the outlet opening (), and a middle portion () between the inlet portion () and the outlet portion (), wherein at least the middle portion () comprises the array of wells () and is provided with a hydrophilic surface, and the flow channel () further comprises a transition area () provided at least between the middle portion () and the outlet portion (), which transition area () is constituted by providing a hydrophobic surface. Furthermore, a method for manufacturing such a microfluidic device is also provided.

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Zurich, Switzerland

Alexander Meier