Roche Sequencing Solutions, Inc.

Kapa Biosystems, Inc.

Signature Diagnostics Gmbh

Alexander Lovejoy

Bronwen Miller

Brian Christopher Godwin

Joseph Platzer

Daniel Burgess

Jo-Anne Elizabeth Penkler

Bernd Hinzmann

John Palma

John Lee

Stephanie Yaung

Martin Ranik

Sophie Beckert

Lijing Yao

Victoria Constandse

Daniel Klass

Ulrich-Peter Rohr

Melissa Loyzer

The present disclosure provides a method for enrichment of at least one target nucleic acid in a library of nucleic acids. A first oligonucleotide is hybridized to a target nucleic acid in library of nucleic acids having first and second adapters. The hybridized first oligonucleotide is extended with a first polymerase, thereby producing a first primer extension complex including the target nucleic acid and the extended first oligonucleotide. The first primer extension complex is captured, enriched relative to the library of nucleic acids, and a second oligonucleotide is hybridized to the target nucleic acid. The hybridized second oligonucleotide is extended with a second polymerase, thereby producing a second primer extension complex including the target nucleic acid and the extended second oligonucleotide, and further liberating the extended first oligonucleotide from the first primer extension complex.

Target enrichment by unidirectional dual probe primer extension

Present disclosure provides a method including isolating DNA from a source, thereby providing a composition including the isolated DNA. The isolated DNA has at least first and second target regions, where the length of the second target region is greater than the length of the first target region. The method further includes quantifying a total mass of the isolated DNA, quantifying a first quantification cycle (C) of the first target region and a second Cof the second target region, and calculating a Q-ratio for the isolated DNA by dividing the second Cby the first C. The method further includes determining a value for a quality-mass constant (k), estimating a required input mass by dividing kby the Q-ratio, and preparing the isolated DNA for sequencing if the total mass of the isolated DNA in the composition is equal or greater than the required input mass.

System and method for isolation and qualification of nucleic acids

The invention is a method of predicting response to therapy in a colorectal cancer patient, the method comprising analysis of circulating tumor DNA from a patient's sample.

Non-invasive test to predict response to therapy in colorectal cancer patients

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Newark, CA, United States of America

Alexander Lovejoy