Ajinomoto Co., Ltd.

Alexander Konstantinovich Sokolov

Nelli Isaakovna Zhdanova

Vladimir Georgievich Debabov

Nikolai Kazimirovich Yankovsky

Uri Dmitrievich Tsigankov

Andrei Yurevich Christoserdov

Michail Markovich Gusyatiner

Tatiana Alexandrovna Bachina

Vytalyi Arkadievich Lifshits

Sergi Vladimirovich Mashko

Oksana Fedorovna Gavrilova

Evgenyi Moiseevich Khurges

Oleg Alexandrovich Rodionov

Uri Ivanovich Kozlov

Alla Lvovna Lapidus

The present invention provides a novel microbial strain and a method for effectively producing L-threonine. The novel strain can grow on a medium containing molasses, a much cheaper raw material than sucrose. The exemplary novel strain E. coli BKIIM B-5318 bears the plasmid pPRT614, which has threonine biosynthesis genes (thr A, B and C), the expression of which is regulated by a lambda-phage PR promoter and temperature-sensitive C1 repressor. The present microorganism is prototrophic with regard to isoleucine. The strain E. coli BKIIM B-5318 produces more than 70 g/l of L-threonine when cultured at 38-41.degree. C. for 32 hours in a medium containing molasses.

L-threonine-producing microbacteria and a method for the production of

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Moscow, Russia

Alexander Konstantinovich Sokolov