Cardea Bio, Inc.

Ammr Joint Venture

Alexander Kane

Brett R Goldsmith

Kiana Aran

Regis Peytavi

Regis Paytavi

A system and apparatus for direct or indirect target substance signal measurement include an integrated circuit with an array of 2D FETs with corresponding 2D transistor channels and a gate area for receiving a volume of liquid with one or more chemical or biological target substances, a conductive source electrically coupled to a first end of the 2D transistor channel, a conductive drain electrically coupled to a second end of the 2D transistor channel, a ceramic coating over the conductive source and the conductive drain and a thin film layer of synthetic biopolymer specific binding agents is adsorbed to the top surface of the 2D transistor channel. Methods for the system and apparatus and for selecting the synthetic biopolymer specific binding agents based on absorptivity are disclosed.

Integrated circuit with 2D FETs for direct and indirect target signal measurement

For enhanced selection of efficient targeted genome manipulating agents, an apparatus includes first and second chip-based biosensors having one or more sensing surfaces configured to detect biomolecular binding interactions between a nucleic acid sample and a targeted genome manipulating agent functionalized to a capture surface within a sensing range of the one or more sensing surfaces. The first chip-based biosensor uses a nucleic acid sample incubated with a blocking agent that blocks on-target binding and the second chip-based biosensor holds a nucleic acid sample that omits the blocking agent. A measurement apparatus measures first and second sets of response signals produced in response to the biomolecular binding interactions occurring between the nucleic acid sample and the targeted genome manipulating agent. An analysis module determines the genome manipulating efficiency parameters of the targeted genome manipulating agent. A system and a method perform the functions of the apparatus.

Enhanced selection of efficient targeted genome manipulating agents

Apparatuses, systems, and methods are disclosed for target detection based on collateral cleavage of a reporter by an enzyme. A biologically gated transistor may include a channel and a reporter moiety immobilized to the channel. The state of the reporter moiety may affect one or more output signals from the biologically gated transistor when excitation conditions are applied to the biologically gated transistor and a sample fluid is applied in contact with the channel. A sample fluid may include an enzyme configured to activate in response to a target nucleic acid to cleave the reporter moiety. Excitation circuitry may apply the excitation conditions, and measurement circuitry may measure output signals from the biologically gated transistor. An analysis module may determine a parameter relating to presence of the target nucleic acid, based on the one or more measurements.

Growing community of inventors

Santa Cruz, CA, United States of America

Alexander Kane