Cornell Research Foundation Inc.

University of Pennsylvania

Cornell University

Androvia Lifesciences, LLC

Tetmedical, Inc.

Alexander J Travis

Gregory S Kopf

Alana J Simpson

Cristina Cardona

G Charles Ostermeier

Melissa A Moody

David R Fischell

Gianpiero D Palermo

Furkat Mukhtarov

John Mcguire Jaicks

Roy Cohen

A coupled enzyme reaction point of care assay system detects and measures an active enzyme biomarker from a patient's blood sample. The assay system has a point of care blood collector for collecting blood into a blood collection vial. A biomarker detection mechanism has at least three fluid flow entities. Each fluid flow entity has a fluid input zone and a corresponding reaction zone. The fluid flow entities include a test strip, a negative control strip and a positive control strip. The reaction zones include components from a multiplicity of substrates, co-factors, buffers and cryoprotectants, and a multiplicity of tethered enzymes. At least one of the tethered enzymes is an enzyme that produces luminescence. The tethered enzymes are adapted to react to a constituent which may be a biomarker. A photonic luminescence reader measures light emitted from an active enzyme biomarker present in the patient's blood.

Apparatus and methods using tethered enzymes for the detection of the enzymatic activity of biomarkers

This disclosure provides a method for determining male fertility status. The method comprises determining Glocalization patterns following induced sperm capacitation, identifying the percentage of various patterns, particularly the ratio of [(AA+APM)/total number of Glocalization patterns] and determining if the percentage of certain Glocalization patterns in response to induced capacitation is altered. Based on the change in the percentage of localization patterns of certain patterns in response to induced capacitation, alone or in combination with other sperm attributes, male fertility status can be identified.

Methods and test kits for determining male fertility status

The present invention relates to a system for production of ATP. This system is comprised of a support and one or more enzymes coupled to that support which are capable of collectively producing ATP from glucose or fructose metabolism. The present invention is additionally directed to a device, which includes the system, and to a method for carrying out a reaction involving the conversion of ATP to ADP using the system.

Method and system for production of adenosine triphosphate

This disclosure provides a method for determining male fertility status. The method comprises determining Gdistribution patterns following induced sperm capacitation, identifying the frequency of distribution of various patterns, and determining if the frequency distribution of certain Gpatterns in response to induced capacitation is altered. Based on the change in the frequency distribution patterns of certain patterns in response to induced capacitation, alone or in combination with other sperm attributes, male fertility status can be identified.

Identifying status of male fertility by determining sperm capacitation

System for production of adenosine triphosphate

This invention describes unique patterns of distribution of ganglioside Gin non-capacitated sperm and demonstrates that the pattern of distribution of Gundergoes changes that can be correlated with the process of capacitation and/or with acrosomal exocytosis. Accordingly, the present invention discloses a method for determining the ability of sperm to respond to capacitation and/or acrosomal exocytosis stimuli. The method comprises determination of distribution pattern for G. The method can be used for both diagnostic and predictive purposes when assessing male reproductive fitness, and can also be used to assess the effects of any agent or environment on sperm including cryoprotective agents and protocols, and contraceptive agents.

Method of determining sperm capacitation

This invention describes unique patterns of distribution of ganglioside Gin non-capacitated sperm and demonstrates that the pattern of distribution of Gundergoes changes that can be correlated with the process of capacitation and/or with acrosomal exocytosis. Accordingly, the present invention discloses a method for determining the ability of sperm to respond to capacitation and/or acrosomal exocytosis stimuli. The method comprises determination of distribution pattern for G. The method can be used for both diagnostic and predictive purposes when assessing male reproductive fitness, and can also be used to assess the effects on sperm of cryoprotective agents and protocols, and contraceptive agents.

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Ithaca, NY, United States of America

Alexander J Travis