Life Technologies Corporation

Berkeley Lights, Inc.

Bruker Cellular Analysis, Inc.

Alexander J Mastroianni

Jeremy Gray

Marc Glazer

Wolfgang Hinz

James A Ball

Christina E Inman

Phil Waggoner

Randall D Lowe, Jr

Ronald L Cicero

Yufang Wang

David Cox

Scott Parker

Kimberly Gorrell

Scott C Benson

Jason M McEwen

Mark P White

David Light

Kristin G Beaumont

Steven Menchen

Barnett B Rosenblum

Carl Williams Fuller

Dmitriy Gremyachinskiy

Paul M Kenney

Roman Rozhkov

Shifeng Li

Shaheer Khan

Jian Gong

Prasanna Krishnan Thwar

Kevin T Chapman

Luisa Andruzzi

Volker L S Kurz

Xiaohua Wang

Gregory G Lavieu

Justin K Valley

Craig Stolarczyk

Diem Tran

Michael L Minto

Peter J Beemiller

Tanya Sokolsky

Natalie C Marks

Kellen C Mobilia

Guido K Stadler

Joseph Koscinski

Shao Ning Pei

John Scott

Yelena Bronevetsky

Mohammad Alanjary

Joshua J Cardiel Rivera

Annamaria Mocciaro

Jason M Mcewen

Amanda L Goodsell

Philip Waggoner

Duane Smith

Geir Fonnum

Astrid Evenroed Molteberg

Grete Modahl

Nini Kjus

Paul Kenney

Wofgang Hinz

M Talha Gokmen

Randall D Lowe

Jr Randall D Lowe

Kevin D Loutherback

Andrew W Mcfarland

Jo Aaserud

A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

Surface treatment of semiconductor sensors

In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.

Covalently modified surfaces, kits, and methods of preparation and use

In biosciences and related fields, it can be useful to study cells in isolation so that cells having unique and desirable properties can be identified within a heterogenous mixture of cells. Processes and methods disclosed herein provide for encapsulating cells within a microfluidic device and assaying the encapsulated cells. Encapsulation can, among other benefits, facilitate analyses of cells that generate secretions of interest which would otherwise rapidly diffuse away or mix with the secretions of other cells.

Methods for encapsulating and assaying cells

A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

Surface stabilization of biosensors

In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces of wells within a well plate to activate lymphocytes, including but not limited to T lymphocytes, in a controllable and reproducible manner.

Functionalized well plate, methods of preparation and use thereof

Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section having a dielectrophoresis configuration; systems that include microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the aqueous droplet; related kits; and methods of manipulating droplets within the microfluidic devices.

Microfluidic apparatus having an optimized electrowetting surface and related systems and methods

A method of forming a polymer matrix array includes treating a surface within a well of a well array with a surface compound including a surface reactive functional group and a radical-forming distal group; applying an aqueous solution including polymer precursors to the well of the well array; and activating the radical-forming distal group of the surface coupling compound with an initiator and atom transfer radical polymerization (ATRP) catalyst to initiate radical polymerization of the polymer precursors within the well of the well array to form the polymer matrix array.

Matrix arrays and methods for making same

A method of preparing a discrete polymer network array include mixing a plurality of nucleic acid polymer networks with a plurality of color-activated polymer networks to form a dispersion, applying the dispersion to an array of wells, the nucleic acid polymer networks selectively depositing into wells of the array of wells, and rinsing the array of wells to selectively remove the plurality of color-activated polymer networks.

Method of distributing discrete polymer networks

Growing community of inventors

Alameda, CA, United States of America

Alexander J Mastroianni