The United States of America, As Represented by the Secretary, Department of Health and Human Services

University of Florida Research Foundation, Incorporated

Cornell Research Foundation Inc.

Centers for Disease Control and Prevention

Intervet International B.v.

Alexander I Klimov

Nancy J Cox

Jacqueline M Katz

Mark William Mellencamp

Edward Joseph Dubovi

Melissa Anne Lum

Daniel Ghislena Emiel Goovaerts

Nallakannu P Lakshmanan

Ruben Omar Donis

Patti Cynthia Crawford

William L Castleman

Paul J Gibbs

Stephen Lindstrom

Lamorris Loftin

Edward J Dubovi

Ruben O Donis

A Michael Frace

Patti C Crawford

Jacqueline Katz

Daniel Ghislena Goovaerts

The subject invention pertains to isolated influenza virus that is capable of infecting canids and causing respiratory disease in the canid. The subject invention also pertains to compositions and methods for inducing an immune response against an influenza virus of the present invention. The subject invention also pertains to compositions and methods for identifying a virus of the invention and diagnosing infection of an animal with a virus of the invention.

Materials and methods for respiratory disease control in canines

Methods of detecting influenza, including differentiating between type and subtype are disclosed, for example to detect, type, and/or subtype an influenza infection. A sample suspected of containing a nucleic acid of an influenza virus, is screened for the presence or absence of that nucleic acid. The presence of the influenza virus nucleic acid indicates the presence of influenza virus. Determining whether the influenza virus nucleic acid is present in the sample can be accomplished by detecting hybridization between an influenza specific probe, influenza type specific probe, and/or subtype specific probe and an influenza nucleic acid. Probes and primers for the detection, typing and/or subtyping of influenza virus are also disclosed. Kits and arrays that contain the disclosed probes and/or primers also are disclosed.

Probe and method for detection and discrimination of types and subtypes of influenza viruses

Primers and probes for detection and discrimination of types and subtypes of influenza viruses

The present invention provides a method of increasing the recombinant expression and solubility of influenza A virus M2 polypeptide comprising nucleic acids encoding variants of the M2 protein of influenza A virus in which transmembrane and other hydrophobic domains have been deleted. The present invention also provides purified polypeptides encoded by the nucleic acids, which polypeptides are immunogenic and are less hydrophobic than full-length M2. Also provided are vaccines comprising variants of M2 expressed in prokaryotic hosts. Further provided are methods of preventing influenza A infection using vaccines comprised of variants of M2. Also provided are antibodies raised against the variants of M2, and use of such antibodies in diagnosis and treatment of influenza A infections.

Preparation and use of recombinant influenza A virus M2 construct vaccines

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Atlanta, GA, United States of America

Alexander I Klimov