Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V.

Alexander Gryanik

Thomas Wittenberg

Tobias Bergen

Christian Muenzenmayer

Christian MÜNZENMAYER

Embodiments of the present invention relate to a method for generating a microscopy representation of a three-dimensional sample having a lateral extension in the x and y directions. The method has the following steps: a) arranging the flat three-dimensional sample on a positioner; b) recording the sample by means of an imaging device to obtain a first microscopy picture of the sample having a first section; c) altering the perspective onto the two-dimensional sample in the z direction; d) recording the sample by means of the imaging device to obtain a second microscopy picture of the sample having a second section; e) determining change information which allows drawing conclusions as to the change in perspective in the z direction, using a difference between the first and second microscopy pictures; and f) merging the first and second microscopy pictures while considering the change information to obtain the microscopy panoramic representation.

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Erlangen, Germany

Alexander Gryanik