Nexus Dx, Inc.

Quidel Corporation

Guild Associates, Inc.

Alexander Belenky

Richard Laswell Egan

Graham Peter Lidgard

David Dickson Booker

Christopher Johann Johnson

Stan Vukajlovich

David Alexander Schofield

Conan Li

Ed Brennan

Methods for determining the susceptibility or resistance of bacteria to antibiotic agents are provided. In one embodiment, the methods include culturing the bacteria in the presence or absence or the antimicrobial agent to generate a primary culture which is then cultured in the presence or absence of transforming phages. The recombinant phages are specific to the bacteria and comprise a heterologous marker (e.g., a nucleic acid that is expressible as a detectable product such as an RNA or a protein). The susceptibility or resistance of the bacteria to the antimicrobial agent may be determined by assaying the culture for the presence or absence of the heterologous marker, wherein a reduction in the level or activity of the marker in the culture compared to the level or activity of the marker in a comparative culture indicates that the bacteria is sensitive to the antibiotic agent.

Phage-mediated immunoassay and methods for determining susceptibility of bacteria to antibiotic or probiotic agents

The invention provides compositions, systems and methods for detecting multiple analytes from a sample.

Multianalyte assay

Growing community of inventors

San Diego, CA, United States of America

Alexander Belenky