Biostore New Zealand Limited

Living Cell Products Pty Ltd.

Alexander B Ferguson

Philippa M Wiggins

James Douglas Watson

Robert Bartlett Elliott

Olga Garkavenko

Phillipa M Wiggins

The invention relates to methods of selecting and maintaining a population of pigs having a low copy number of porcine endogenous retrovirus, and the use of such pigs as a source of cells, tissue and/or organs suitable for xenotransplantation. The invention further relates to methods for selecting cells, tissue and/or organs from such pigs for suitability for use in xenotransplantation.

Swine population having low levels of porcine endogenous retrovirus and uses thereof

The present invention provides solutions and methods for preserving living biological materials that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of dihydrogen phosphate, bicarbonate, nitrate, bisulfate and iodide. In one embodiment, preferred for the preservation of platelets, the solutions comprise betaine, sodium chloride and sodium citrate. For the preservation of many living biological materials, the inventive solutions preferably contain a calcium salt selected from the group consisting of calcium sulfate and calcium chloride.

Compositions and methods for the preservation of living tissues

Solutions for the preservation of tissues

The present invention provides solutions and methods for preserving biological material that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of univalent oxyanions and of iodide. In one embodiment, the solutions comprise a first neutral solute having a molecular weight of at least about 335 and a solubility in water of at least about 0.3 M, a second neutral solute having a molecular weight of less than about 200 and having both hydrophilic and hydrophobic moieties, and sodium citrate. The inventive solutions preferably contain a calcium salt selected from the group consisting of calcium sulfate and calcium chloride.

Composition comprising raffinose, TMAO, sodium citrate and methods for

The present invention provides solutions and methods for preserving living biological materials that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of dihydrogen phosphate, bicarbonate, nitrate, bisulfate and iodide. In one embodiment preferred for the preservation of platelets, the solutions comprise betaine, sodium chloride and sodium citrate. For the preservation of many living biological materials, the inventive solutions preferably contain a calcium salt selected from the group consisting of calcium sulfate and calcium chloride.

Compositions comprising betaine, sodium citrate and sodium chloride and

The present invention provides solutions and methods for preserving living biological materials that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of dihydrogen phosphate, bicarbonate, nitrate bisulfate and iodide. In one embodiment, the solutions comprise a first neutral solute having a molecular weight of at least about 335 and a solubility in water of at least about 0.3 M, a second neutral solute having a molecular weight of less than about 200 and having both hydrophilic and hydrophobic moieties, with the first neutral solute preferably being selected from the group consisting of raffinose and trehalose and the second neutral solute preferably being selected from the group consisting of trimethyl amine oxide and betaine. For the preservation of many living biological materials, the inventive solutions preferably contain a calcium salt selected from the group consisting of calcium sulfate and calcium chloride.

The present invention provides solutions and methods for preserving biological material that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of univalent oxyanions and of iodide. The solutions comprise a first neutral solute having a molecular weight of at least about 335 and a solubility in water of at least about 0.3M, and a second neutral solute having a molecular weight of less than about 200 and having both hydrophilic and hydrophobic moieties. The inventive solutions preferably contain CaSO.sub.4, together with combinations of anions and cations from the protein-stabilizing ends of the Hofmeister series, such as K.sub.2 SO.sub.4. Solutions with raffinose or trehalose in combination with trimethyl amine oxide or betaine are particularly preferred.

The present invention provides solutions and methods for preserving biological material that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of univalent oxyanions and of iodide. The solutions comprise a first neutral solute having a molecular weight of at least about 335 and a solubility in water of at least about 0.3M, and a second neutral solute having a molecular weight of less than about 200 and having both hydrophilic and hydrophobic moieties. The inventive solutions preferably contain CaSO.sub.4, together with combinations of anions and cations from the protein-stabilizing ends of the Hofmeister series, such as K.sub.2 SO.sub.4. In particular methods using solutions comprising raffinose or trehalose and trimethyl amine oxide or betaine, wherein the solution is substantially isotonic with the biological material and the solution is substantially free of iodide, dihydrogen phosphate, bicarbonate, nitrate and bisulfate are described.

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Auckland, New Zealand

Alexander B Ferguson