Qiagen Gmbh

Novartis Ag

Ehrfeld Mikrotechnik Bts Gmbh

Pergan Hilfsstoffe Fur Industrielle Prozesse Gmbh

Alexander Azzawi

Ralf Peist

Troy Vernon Holland

Dawn A Smith

Holger Engel

Jie Kang

Francesca Di Pasquale

Peter Hahn

Brian Gerrard Devlin

Eric Lader

Hans-Ernst Mehesch

Peter Grünefeld

Wolfgang Bielke

Martin Sondermann

Edgar Von Zadow

Stefan Kirsch

Bernhard Wormland

Dawn Alison Smith

The invention is in the field of regulation of enzymatic activity in nucleic acid modifying reactions. It describes a method of regulating enzymatic activity by adding chelating agents to the reaction composition and exploits the fact that both the binding of divalent cations to these chelating agents and the pH of commonly used buffers is temperature dependent. PCR experiments that are hampered by non-specific side products can be regulated such that the target sequence is amplified in a more specific manner.

Cation chelator hot start

Caution chelator hot start

The present invention pertains to the use of at least one abasic modification within the first 8 nucleotide positions of the 5' region of the antisense strand of a small interfering nucleic acid (siNA) molecule for reducing off-target effects. Provided are suitable modified siNAs, compositions and methods for producing respective siNAs, as well as kits comprising respective siNAs.

Modified siNA

The invention provides a process for efficient and reliable preparation of organic peroxides, preferably dialkyl peroxides, peroxycarboxylic acids, peroxycarboxylic esters, diacyl peroxides, peroxycarbonate esters, peroxydicarbonates, ketone peroxides and perketals with the aid of at least one static micromixer and an apparatus for performing the process.

Method for the production of organic peroxides by means of a microreaction technique

The invention provides a continuous-flow process for producing a polymeric material. The process of the invention comprises the steps of: introducing at least two solutions, each comprising one or more reactants, into a microreactor to mix the two solutions to form a reacting solution, wherein the one or more reactants are selected from the group consisting of a monomer, an initiator, a coupling agent, a prepolymer, a macromer, and mixtures thereof; feeding continuously the reacting solution into a flow-through polymerization reactor in a coil form, wherein the flow-through polymerization reactor is capable of providing a residence time sufficient for (co)polymerizing the reactants to form a polymer with a desired polydispersity, wherein the flow-through polymerization reactor is immersed in an ultrasonic bath to minimize or substantially eliminate unwanted high-molecular-weight fractions of the polymer.

Continuous process for production of polymeric materials

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Solingen, Germany

Alexander Azzawi