Cepheid Corporation

Epoch Biosciences, Inc.

Epoch Pharmaceuticals, Inc.

Koronis Pharmaceuticals, Inc.

Elitech Holding B.v.

Molecular Imaging Corporation

Microprobe, Inc.

Elitech Holdings B.v.

1,3,5-triazines for Treatment of Viral Diseases

Alexander A Gall

Igor V Kutyavin

Nicolaas M J Vermeulen

Robert O Dempcy

Eugeny Alexander Lukhtanov

Rich B Meyer, Jr

Richard Daifuku

Michael W Reed

Irina Aleksandrovna Afonina

Kevin P Lund

Sergey Lokhov

Howard B Gamper

Dmitri S Sergueev

Sergey G Lokhov

Michael J Singer

Kevin Harris

Dina Sologub

Ekaterina V Viazovkina

Dmitri Sergueev

Vera Baraznenok

Stuart Lindsay

Tianwei Jing

Russell G Higuchi

David H Persing

Mikhail A Podyminogin

Andrew Kohlway

Alex I Kutyavin

Oliver Z Nanassy

Yuri L Lyubchenko

Ling Li

Charles R Petrie

John C Tabone

Reuel Van Atta

Yevgeniy S Belousov

Irina Shishkina

Kriszten Kocmond

Edwin Wei-Lung Lai

Gerald D Hurst

Arthur D Broom

Reuel Vanatta

Edwin W Lai

William Brabant

Karen M Singer, Legal Representative

Ivan Baraznenok

Maher N Qabar

Nicolaas MJ Vermeulen

Non-ionic polyether surfactants with improved resistance to hydrolysis are provided.

Nonionic polyether surfactants

The present disclosure relates to aptamers for temperature-dependent reversible inhibition of thermostable polymerase activity in order to improve sensitivity and specificity of various reactions and assays involving hot start polynucleotide synthesis. Methods for use of the aptamers and related compositions and kits are also provided.

Thermostable polymerase inhibitor compositions and methods

Methods and compositions for isolation of nucleic acids from biological samples are provided.

Methods and compositions for nucleic acid isolation

Automated oligonucleotide synthesis-compatible sulforhodamine dye phosphoramidite compounds and labeled polynucleotides incorporating these dyes are provided.

Sulforhodamine phosphoramidite dyes

Methods and cleaning compositions for reduction of nucleic acid contamination on surfaces, in air, and in solutions using modified pectin are provided.

Nucleic acid decontamination methods

Automated oligonucleotide synthesis-compatible fluorescent dye phosphoramidite compounds, solid supports, and labeled polynucleotides incorporating the compounds are provided. The compounds allow universal incorporation of the fluorescent label into any position of the polynucleotide.

Fluorescent dyes

In various embodiments methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulfonating the bound deaminated nucleic acid and/or simultaneously eluting and desulfonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.

Integrated purification and measurement of DNA methylation and co-measurement of mutations and/or MRNA expression levels in an automated reaction cartridge

Methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and/or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulphonating the bound deaminated nucleic acid and/or simultaneously eluting and desulphonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and/or nucleic acid sequencing, and/or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.

Integrated purification and measurement of DNA methylation and co-measurement of mutations and/or mRNA expression levels in an automated reaction cartridge

Disclosed are modified cytosine bases that provide enhanced base-pairing affinity for guanine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified cytosine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection.

Modified cytosine polynucleotide oligomers and methods

Disclosed are modified thymine bases that provide enhanced base-pairing affinity for adenine or 2,6-diaminopurine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified thymine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection.

Modified thymine polynucleotide oligomers and methods

The present invention relates to novel polar fluorescent and quenchers dyes, and minor groove binder with enhanced polarity. The present invention further relates to methods of preparing oligonucleotide probes labeled with polar arsonate dyes under the condition of automated synthesis and method of using such probes.

Polar dyes

The present invention provides hydrophobic prodrugs of bases, nucleosides, and nucleotides as well as methods of using the prodrugs as antiviral and anti-cancer chemotherapeutic agents.

Prodrugs of heteroaryl compounds

Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.

Fluorescent quenching detecting reagents and methods

Growing community of inventors

Woodinville, WA, United States of America

Alexander A Gall